EXPRESSION OF CDX-2 IN THE MOUSE EMBRYO AND PLACENTA - POSSIBLE ROLE IN PATTERNING OF THE EXTRAEMBRYONIC MEMBRANES
EXPRESSION OF CDX-2 IN THE MOUSE EMBRYO AND PLACENTA - POSSIBLE ROLE IN PATTERNING OF THE EXTRAEMBRYONIC MEMBRANES
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DOI：
10.1002/aja.1002040302
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发表时间：
1995-11-01
影响因子：
2.5
通讯作者：
JAMES, R
中科院分区：
文献类型：
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作者：
BECK, F;ERLER, T;JAMES, R
果蝇同源异型基因Caudal（Cad）的三个小鼠同源物已被描述。它们目前被命名为Cdx-1、Cdx-2和Cdx-4，Cdx-1和2都在成体中肠和后肠中强烈表达，而Cdx-1和4已被证明在胚胎原条中被激活。使用针对含有鼠Cdx-2的氨基末端109个氨基酸的融合蛋白的多克隆抗体，我们在这里描述了从早期卵裂到胚胎发育12.5天的基因产物的地形位置。Cdx-2表达开始于3.5天，并局限于滋养外胚层，不存在于内细胞团。随后，染色位于邻近上胚层的胚外胚层，但保留了更表面放置的极细胞以及壁滋养层细胞。在胎膜中的持续表达涉及绒毛膜、尿囊芽，甚至在更晚的阶段，涉及海绵滋养层。从8.5天开始，Cdx-2开始在胚胎组织中表达，主要（与Cdx-1不同）在最早形成的肠的后部，以及在尾芽和神经管的尾部。Cdx-2，因此，转录以及之前的任何其他膜的Cad同源物组和相关的Hox-C组;其在胚外膜和后肠中的表达反映了泄殖腔和绒毛膜尿囊之间的系统发育关系，并表明同源异型盒基因可能参与胎盘发育和/或图案化的可能性。(C)1995 Wiley-Liss，Inc.
Three mouse homologues of the Drosophila homeotic gene Caudal (Cad) have been described. They are currently designated Cdx-1, Cdx-2, and Cdx-4, Cdx-1 and 2 are both strongly expressed in the adult mid- and hindgut, while Cdx-1 and 4 have been shown to be activated in the embryonic primitive streak, Using a polyclonal antibody against a fusion protein containing the amino terminal 109 amino acids of murine Cdx-2, we here describe the topographical location of the gene product from early cleavage to 12.5 days of embryonic development. Cdx-2 expression begins at 3.5 days and is confined to the trophectoderm, being absent from the inner cell mass. Subsequently, staining is located in the extra-embryonic ectoderm adjacent to the epiblast, but sparing the more superficially placed polar, as well as the mural trophoblastic cells. Continuing expression in the fetal membranes involves the chorion, the allantoic bud, and, at even later stages, the spongiotrophoblast. From 8.5 days, Cdx-2 begins to be expressed in embryonic tissues, principally (unlike Cdx-1) in the posterior part of the gut from its earliest formation, as well as in the tail bud and in the caudal part of the neural tube. Cdx-2 is, therefore, transcribed well before any other membrane of the Cad homologue group and of the related Hox-C group; its expression in the extra-embryonic membranes and in the hindgut reflects the phylogenetic relationship between the cloaca and the chorio-allantois and suggests the possibility that homeobox genes may be involved in placental development and/or patterning. (C) 1995 Wiley-Liss, Inc.