Detection and identification of genetic material via single-molecule conductance
Detection and identification of genetic material via single-molecule conductance
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DOI：
10.1038/s41565-018-0285-x
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发表时间：
2018-12-01
影响因子：
38.3
通讯作者：
Hihath, Joshua
中科院分区：
文献类型：
--
作者：
Li, Yuanhui;Artes, Juan M.;Hihath, Joshua
RNA模式的持续发现（例如，非编码，微型和增强剂）导致人们对检测，测序和识别用于食品安全，水和环境保护，动植物病理学，临床诊断和研究以及生物率的RNA段的渴望增加了。在这里，我们证明了单分子电导技术可用于从短RNA寡核苷酸中提取与生物学相关的信息，这些测量值对attomolar靶标的浓度敏感，它们能够被多重多发，并且可以检测到其他可能存在的，可能会干扰，RNA序列的目标。我们还证明了RNA的电荷转运特性：DNA杂交对单核苷酸多态性敏感，从而使大肠杆菌的特定血清型之间有分化。使用光谱和计算方法的组合，我们确定电导敏感性主要来自突变对分子构象结构的影响，而不是由直接的化学替代物产生的。我们认为，可以进一步开发这种方法以制造一种基于电的传感器来诊断目的。
The ongoing discoveries of RNA modalities (for example, non-coding, micro and enhancer) have resulted in an increased desire for detecting, sequencing and identifying RNA segments for applications in food safety, water and environmental protection, plant and animal pathology, clinical diagnosis and research, and bio-security. Here, we demonstrate that single-molecule conductance techniques can be used to extract biologically relevant information from short RNA oligonucleotides, that these measurements are sensitive to attomolar target concentrations, that they are capable of being multiplexed, and that they can detect targets of interest in the presence of other, possibly interfering, RNA sequences. We also demonstrate that the charge transport properties of RNA:DNA hybrids are sensitive to single-nucleotide polymorphisms, thus enabling differentiation between specific serotypes of Escherichia coli. Using a combination of spectroscopic and computational approaches, we determine that the conductance sensitivity primarily arises from the effects that the mutations have on the conformational structure of the molecules, rather than from the direct chemical substitutions. We believe that this approach can be further developed to make an electrically based sensor for diagnostic purposes.