Molecular cloning and mRNA expression of cytochrome P4501A1 and 1A2 in the liver of common minke whales (Balaenoptera acutorostrata).
Molecular cloning and mRNA expression of cytochrome P4501A1 and 1A2 in the liver of common minke whales (Balaenoptera acutorostrata).
复制标题
常见小须鲸 (Balaenoptera acutorostrata) 肝脏中细胞色素 P4501A1 和 1A2 的分子克隆和 mRNA 表达。
DOI：
10.1016/j.marpolbul.2005.07.012
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发表时间：
2005
影响因子：
5.8
通讯作者：
S. Tanabe
中科院分区：
文献类型：
--
作者：
Satoko Niimi;Michio X. Watanabe;Eun;H. Iwata;Genta Yasunaga;Y. Fujise;S. Tanabe
本研究展示了北太平洋小须鲸 (Balaenoptera acutorostrata) CYP1A1 和 1A2 的全长 cDNA 序列。 CYP1A1和CYP1A2 cDNA均具有516个氨基酸残基的开放阅读框，预测分子量分别为58.3kDa和58.1kDa。推导的CYP1A1全长氨基酸序列与绵羊(86%)和猪(87%)的同源性较高，而CYP1A2的全长氨基酸序列与迄今为止分离出的CYP1A2物种中的人类(82%)和猴CYP1A2(82%)关系最为密切。普通小须鲸和其他哺乳动物物种之间 CYP1A1 和 1A2 某些保守和功能性氨基酸残基的差异表明它们具有特定代谢功能的可能性。在普通小须鲸肝脏中分析的包括 PCB 和 DDT 在内的有机氯化合物 (OC) 浓度与肝脏 CYP1A1 和 CYP1A2 mRNA 表达水平没有显着相关性，表明此类 OC 不会诱导这些酶。
This study presents full-length cDNA sequences of CYP1A1 and 1A2, in common minke whale (Balaenoptera acutorostrata) from the North Pacific. Both CYP1A1 and CYP1A2 cDNAs had an open reading frame of 516 amino acid residues, and predicted molecular masses were 58.3kDa and 58.1kDa, respectively. The deduced full-length amino acid sequence of CYP1A1 revealed higher identities with those of sheep (86%) and pig (87%), and that of CYP1A2 was most closely related to human (82%) and monkey CYP1A2 (82%) among species from which CYP1A2 has been isolated so far. Differences in certain conserved and functional amino acid residues of CYP1A1 and 1A2 between common minke whale and other mammalian species indicate the possibility of their specific metabolic function. Concentrations of organochlorine compounds (OCs) including PCBs and DDTs analyzed in common minke whale liver showed no significant correlation with hepatic mRNA expression levels of CYP1A1 and CYP1A2, indicating no induction of these enzymes by such OCs.
白鲸 (Delphinapterus leucas) 肝微粒体细胞色素 P450 的催化和免疫化学特征。
影响因子：
3.8
作者：
White,RD;Hahn,ME;Lockhart,WL;Stegeman,JJ
通讯作者：
Stegeman,JJ