Activity Increase in Respiratory Chain Complexes by Rubella Virus with Marginal Induction of Oxidative Stress
Activity Increase in Respiratory Chain Complexes by Rubella Virus with Marginal Induction of Oxidative Stress
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DOI：
10.1128/jvi.00533-13
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发表时间：
2013-08-01
影响因子：
5.4
通讯作者：
Liebert, U. G.
中科院分区：
文献类型：
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作者：
Claus, C.;Schoenefeld, K.;Liebert, U. G.
线粒体对病毒的生命周期很重要，主要是通过提供病毒复制和组装所需的能量。风疹病毒(RV)与线粒体发生高度复杂的相互作用，其中包括线粒体膜电位的增加，作为线粒体活性的一般标志。本研究旨在更全面地了解线粒体呼吸链复合体I至IV的活性，并对其在三种不同细胞系中的活性进行比较。线粒体呼吸酶琥珀酸氧化还原酶(复合体II)和细胞色素c氧化还原酶(复合体III)活性均有不同程度的升高。而线粒体呼吸酶细胞色素C氧化酶(复合体IV)活性则显著降低。对线粒体功能的影响似乎是轮状病毒特有的，因为它们在控制麻疹病毒感染时是不存在的。此外，呼吸链活性的这些变化与氧化应激蛋白转录的升高无关，并且只诱导了少量的活性氧物种(ROS)。此外，线粒体生物发生和结构标志物的蛋白质和/或mRNA水平升高，如核呼吸因子(NRFs)和丝裂原蛋白2(Mfn2)。综上所述，这些结果为病毒对线粒体功能的调控建立了一个新的观点。
Mitochondria are important for the viral life cycle, mainly by providing the energy required for viral replication and assembly. A highly complex interaction with mitochondria is exerted by rubella virus (RV), which includes an increase in the mitochondrial membrane potential as a general marker for mitochondrial activity. We aimed in this study to provide a more comprehensive picture of the activity of mitochondrial respiratory chain complexes I to IV. Their activities were compared among three different cell lines. A strong and significant increase in the activity of mitochondrial respiratory enzyme succinate: ubiquinone oxidoreductase (complex II) and a moderate increase of ubiquinol: cytochrome c oxidoreductase (complex III) were detected in all cell lines. In contrast, the activity of mitochondrial respiratory enzyme cytochrome c oxidase (complex IV) was significantly decreased. The effects on mitochondrial functions appear to be RV specific, as they were absent in control infections with measles virus. Additionally, these alterations of the respiratory chain activity were not associated with an elevated transcription of oxidative stress proteins, and reactive oxygen species (ROS) were induced only marginally. Moreover, protein and/or mRNA levels of markers for mitochondrial biogenesis and structure were elevated, such as nuclear respiratory factors (NRFs) and mitofusin 2 (Mfn2). Together, these results establish a novel view on the regulation of mitochondrial functions by viruses.