Evaluation of the utility of interphase cytogenetics to detect residual cells with a malignant genotype in mixed cell populations: a Burkitt lymphoma model.
Evaluation of the utility of interphase cytogenetics to detect residual cells with a malignant genotype in mixed cell populations: a Burkitt lymphoma model.
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评估间期细胞遗传学在检测混合细胞群中具有恶性基因型的残留细胞的效用：伯基特淋巴瘤模型。
DOI：
10.1089/dna.1993.12.637
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发表时间：
1993
影响因子：
3.1
通讯作者：
Ward,DC
中科院分区：
文献类型：
--
作者：
Ried,T;Lengauer,C;Lipp,M;Fischer,C;Cremer,T;Ward,DC
间期细胞遗传学已被用于检测存在大量过量正常细胞的肿瘤细胞。选择荧光原位杂交探针来揭示肿瘤细胞核中特定的杂交模式，并为杂交结果的评估提供内部控制。通过列举来自伯基特淋巴瘤细胞系的细胞遗传学正常细胞和肿瘤细胞的混合物，我们能够以500分之一的频率检测到肿瘤细胞。正常细胞可以从伯基特淋巴瘤细胞中分化出来，特异性为99.9%。
Interphase cytogenetics has been used to detect tumor cells in the presence of a large excess of normal cells. Probes for fluorescencein situhybridization were chosen to reveal a specific hybridization pattern in tumor cell nuclei as well as to provide an internal control for the assessment of the hybridization results. By enumerating mixtures of cytogenetically normal cells and tumor cells from a Burkitt lymphoma cell line, we were able to detect tumor cells at a frequency of one in 500. Normal cells could be differentiated from Burkitt lymphoma cells with a specificity of ∼99.9%.