Nuclease treatment enhanced the ameliorative effect of yeast culture on epidermal mucus, hepatic lipid metabolism, inflammation response and gut microbiota in high-fat diet-fed zebrafish
Nuclease treatment enhanced the ameliorative effect of yeast culture on epidermal mucus, hepatic lipid metabolism, inflammation response and gut microbiota in high-fat diet-fed zebrafish
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核酸酶处理增强了酵母培养物对高脂饮食喂养的斑马鱼表皮粘液、肝脏脂质代谢、炎症反应和肠道微生物群的改善作用
DOI：
10.1016/j.fsi.2022.11.025
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Zhigang Zhou
中科院分区：
文献类型：
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作者：
Yajie Zhao;Qiang Hao;Qingshuang Zhang;Yalin Yang;Chao Ran;Qiyou Xu;Chenglong Wu;Wenshu Liu;Siming Li;Zhen Zhang;Zhigang Zhou
作为功能性饲料添加剂，酵母培养物中含有丰富的核苷酸，添加额外的核酸酶可以显著提高酵母培养物中核苷酸的含量。在该实验中，通过向酵母培养物或核酸酶处理的酵母培养物补充高脂饮食（HFD）来评估对斑马鱼的生长、表皮粘液、肝脏和肠道健康的影响。1月龄的斑马鱼被喂食四种饲料：正常饲料（NORM）、HFD、酵母培养物饲料（YC）和核酸酶处理的酵母培养物饲料（YC（N）），为期三周。结果表明，YC（N）组表皮粘液补体4活性显著高于HFD组和YC组（P < 0.05）。YC和YC（N）能显著降低HFD引起的肝组织甘油三酯含量（P < 0.05）。与YC组相比，YC（N）组大鼠肝脏脂肪分解相关基因PPAR α、PGC 1 α、ACOX 3的表达显著增加（P < 0.05）。与YC组比较，YC（N）组肝组织促炎因子TNF α、IL-6、IL-1β和抗炎因子TGF β、IL-10的表达均显著增加（P < 0.05）。YC和YC（N）组肠绒毛高度显著提高（P < 0.05）。与HFD组比较，YC（N）组肠黏膜促炎因子TNF α、IL-6和抗炎因子TGF β、IL-10的表达均显著增加（P < 0.05）。YC（N）组肠道变形菌和不动杆菌的丰度显著降低，而放线菌、分枝杆菌和红杆菌的丰度显著升高（P < 0.05）。与酵母培养物相比，核酸酶处理的酵母培养物能进一步缓解HFD对鱼类肝脏和肠道健康的不良影响，可作为饲料添加剂用于鱼类的营养和免疫调节。
As a functional feed additive, yeast cultures are rich in nucleotides, and adding extra nuclease can significantly increase the content of nucleotides in yeast culture. In this experiment, the effects on growth, epidermal mucus, liver and intestinal health of zebrafish were evaluated by supplementing the yeast culture or nuclease-treated yeast culture with a high-fat diet (HFD). One-month-old zebrafish were fed four diets: normal diet (NORM), HFD, yeast culture diet (YC), and nuclease-treated yeast culture diet (YC (N)) for three weeks. Results showed that the complement 4 activity of the epidermal mucus in YC (N) group was significantly higher than those in HFD and YC groups (P < 0.05). The YC and YC (N) significantly reduced the content of hepatic triglyceride caused by HFD (P < 0.05). Moreover, compared with the YC group, the YC (N) significantly increased the expression of lipolysis genes, such asPPARα, PGC1α, ACOX3(P < 0.05). Compared with the YC group, the YC (N) group significantly increased the expression of liver pro-inflammatory factorsTNFα,IL-6,IL-1βand anti-inflammatory factorsTGFβ,IL-10(P < 0.05). The diet YC and YC (N) significantly improved the height of the intestinal villus (P < 0.05). Compared with the HFD group, the YC (N) group significantly increased the expression of intestinal pro-inflammatory factorsTNFα,IL-6and anti-inflammatory factorsTGFβ,IL-10(P < 0.05). The YC (N) group significantly decreased the abundance of intestinal Proteobacteria and Acinetobacter, and increased the abundance of intestinal Actinobacteria, Mycobacterium and Rhodobacter (P < 0.05). In conclusion, compared with the supplement of yeast culture, nuclease treated yeast culture can further alleviate the adverse effects of HFD on liver and intestinal health, and be used as feed additives for the nutritional and immune regulation of fish.