Evaluation of the histological and mechanical features of tendon healing in a rabbit model with the use of second-harmonic-generation imaging and tensile testing.
Evaluation of the histological and mechanical features of tendon healing in a rabbit model with the use of second-harmonic-generation imaging and tensile testing.
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DOI：
10.1302/2046-3758.511.bjr-2016-0162.r1
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发表时间：
2016-11
影响因子：
4.6
通讯作者：
Yasui T
中科院分区：
文献类型：
--
作者：
Hase E;Sato K;Yonekura D;Minamikawa T;Takahashi M;Yasui T
本研究旨在通过二次谐波成像（SHG）和拉伸试验评价兔肌腱愈合模型的组织学和力学特征。本研究共使用8只雄性日本白色家兔。将其右腿指屈肌腱锐性切断，然后采用腱内缝合修复。术后4周处死动物，取左右腿趾屈肌腱，分别作为肌腱愈合标本（n = 8）和对照标本（n = 8）。通过SHG成像检查每个样本，然后进行拉伸测试，并评估两种测试模式的结果的相关性。虽然愈合肌腱样品的SHG光强度显著低于未受伤肌腱样品的SHG光强度，但2D傅立叶变换SHG图像显示未受伤和愈合样品之间以及愈合样品之间的胶原纤维结构存在明显差异。SHG图像的平均强度显示出与从拉伸测试获得的杨氏模量的中等相关性（R2 = 0.37）。我们的研究结果表明，SHG显微镜可能是肌腱愈合的潜在指标。引用这篇文章：E。Hase，K. Sato，D.米仓，T. Minamikawa，M. Takahashi，T.安井利用二次谐波成像和拉伸试验评价兔模型中肌腱愈合的组织学和力学特征。骨关节研究2016;5：577-585。DOI：10.1302/2046-3758.511.BJR-2016-0162.R1。
This study aimed to evaluate the histological and mechanical features of tendon healing in a rabbit model with second-harmonic-generation (SHG) imaging and tensile testing. A total of eight male Japanese white rabbits were used for this study. The flexor digitorum tendons in their right leg were sharply transected, and then were repaired by intratendinous stitching. At four weeks post-operatively, the rabbits were killed and the flexor digitorum tendons in both right and left legs were excised and used as specimens for tendon healing (n = 8) and control (n = 8), respectively. Each specimen was examined by SHG imaging, followed by tensile testing, and the results of the two testing modalities were assessed for correlation. While the SHG light intensity of the healing tendon samples was significantly lower than that of the uninjured tendon samples, 2D Fourier transform SHG images showed a clear difference in collagen fibre structure between the uninjured and the healing samples, and among the healing samples. The mean intensity of the SHG image showed a moderate correlation (R2 = 0.37) with Young’s modulus obtained from the tensile testing. Our results indicate that SHG microscopy may be a potential indicator of tendon healing. Cite this article: E. Hase, K. Sato, D. Yonekura, T. Minamikawa, M. Takahashi, T. Yasui. Evaluation of the histological and mechanical features of tendon healing in a rabbit model with the use of second-harmonic-generation imaging and tensile testing. Bone Joint Res 2016;5:577–585. DOI: 10.1302/2046-3758.511.BJR-2016-0162.R1.