The coordinate regulation of pharyngeal development in C-elegans by lin-35/Rb, pha-1, and ubc-18
The coordinate regulation of pharyngeal development in C-elegans by lin-35/Rb, pha-1, and ubc-18
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DOI：
10.1016/j.ydbio.2004.03.022
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发表时间：
2004-07-01
影响因子：
2.7
通讯作者：
Johanson, BL
中科院分区：
文献类型：
--
作者：
Fay, DS;Qiu, XH;Johanson, BL
器官发育是一个复杂的过程，涉及细胞增殖、分化和形态发生事件的协调。使用屏幕，以确定在动物发育过程中与lin-35/Rb协调功能的基因，我们已经分离出一个弱的功能丧失（LOF）突变的pha-1，lin-35; pha-1双突变体是有缺陷的，在咽部形态发生的早期步骤，导致异常的咽部结构。alpha-1也是与其它B类合成多叶（SynMuv）基因（包括秀丽隐杆线虫E2 F同源物efl-1）一起合成致死的。报告分析表明，在胚胎发育过程中，PHA-1广泛表达，其功能存在于细胞质中。我们还提供了遗传和表型证据来支持PHA-1，一种新的蛋白质，和UBC-18，一种泛素结合酶，我们以前已经证明在咽部发育过程中与lin-35一起发挥作用，在平行途径中起作用，以调节共同细胞靶点的活性。(C)2004年爱思唯尔公司版权所有
Organ development is a complex process involving the coordination of cell proliferation, differentiation, and morphogenetic events. Using a screen to identify genes that function coordinately with lin-35/Rb during animal development, we have isolated a weak loss-of-function (LOF) mutation in pha-1, lin-35; pha-1 double mutants are defective at an early step in pharyngeal morphogenesis leading to an abnormal pharyngeal architecture. pha-1 is also synthetically lethal with other class B synthetic multivulval (SynMuv) genes including the C elegans E2F homolog, efl-1. Reporter analyses indicate that pha-1 is broadly expressed during embryonic development and that its functions reside in the cytoplasm. We also provide genetic and phenotypic evidence to support the model that PHA-1, a novel protein, and UBC-18, a ubiquitin-conjugating enzyme that we have previously shown to function with lin-35 during pharyngeal development, act in parallel pathways to regulate the activity of a common cellular target. (C) 2004 Elsevier Inc. All rights reserved