Constructing a dense genetic linkage map and mapping QTL for the traits of flower development in Brassica carinata
Constructing a dense genetic linkage map and mapping QTL for the traits of flower development in Brassica carinata
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甘蓝花发育性状密集遗传连锁图谱构建及QTL定位
DOI：
10.1007/s00122-014-2321-z
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发表时间：
2014-07-01
影响因子：
5.4
通讯作者：
Meng, Jinling
中科院分区：
文献类型：
--
作者：
Zou, Jun;Raman, Harsh;Meng, Jinling
构建了一个完整的密集遗传连锁图谱，用于比较基因组分析和花期QTL鉴定。利用DArT-Seq（TM）标记，在双单倍体群体中构建了Brassica carinata （BBCC）的密集连锁图谱。共定位到4031个标记，对应1366个独特位点，包括639个箱，遗传距离为2048 cM。我们鉴定了保存在芸苔科植物中的136个块和岛，它们显示了六倍化的特征，代表了祖先十字花科植物的核型。B. carinata的B和C基因组与B. nigra/B的B基因组共有85%的共同保守区。juncea和80%的一般保护街区的C基因组显著,和频繁的插入和反演等结构性重组。在DH群体中鉴定出了24个与开花和出芽时间有关的数量性状位点（QTL）。在这些QTL中，在B4连锁群的J区所有环境中都鉴定出一个与开花时间有关的一致QTL (qFT.B4-2)，其中一组与开花时间有关的基因在拟南芥中对齐。另一个与冬季作物环境下开花时间有关的主要QTL是在C6的E区发现的，在此之前，bft -C6基因被定位在甘蓝型油菜中。该高密度图谱不仅有助于通过QTL分析和基因组测序揭示该物种的遗传变异，而且还可用于标记辅助选择和基因组选择等其他方面的改良。
An integrated dense genetic linkage map was constructed in a B. carinata population and used for comparative genome analysis and QTL identification for flowering time.An integrated dense linkage map of Brassica carinata (BBCC) was constructed in a doubled haploid population based on DArT-Seq(TM) markers. A total of 4,031 markers corresponding to 1,366 unique loci were mapped including 639 bins, covering a genetic distance of 2,048 cM. We identified 136 blocks and islands conserved in Brassicaceae, which showed a feature of hexaploidisation representing the suggested ancestral crucifer karyotype. The B and C genome of B. carinata shared 85 % of commonly conserved blocks with the B genome of B. nigra/B. juncea and 80 % of commonly conserved blocks with the C genome of B. napus, and shown frequent structural rearrangements such as insertions and inversions. Up to 24 quantitative trait loci (QTL) for flowering and budding time were identified in the DH population. Of these QTL, one consistent QTL (qFT.B4-2) for flowering time was identified in all of the environments in the J block of the B4 linkage group, where a group of genes for flowering time were aligned in A. thaliana. Another major QTL for flowering time under a winter-cropped environment was detected in the E block of C6, where the BnFT-C6 gene was previously localised in B. napus. This high-density map would be useful not only to reveal the genetic variation in the species with QTL analysis and genome sequencing, but also for other applications such as marker-assisted selection and genomic selection, for the African mustard improvement.