In vivo footprinting reveals unique cis-elements and different modes of hypoxic induction in maize Adh1 and Adh2.
In vivo footprinting reveals unique cis-elements and different modes of hypoxic induction in maize Adh1 and Adh2.
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DOI：
10.1105/tpc.3.2.159
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发表时间：
1991-02
期刊：
影响因子：
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通讯作者：
A. Paul;R. Ferl
中科院分区：
文献类型：
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作者：
A. Paul;R. Ferl
玉米醇脱氢酶-1（Adh 1）和醇脱氢酶-2（Adh 2）的转录激活伴随着每个基因的5 '侧翼区域内的染色质结构的变化。通过在低氧诱导8小时内对玉米悬浮培养物进行体内硫酸二甲酯足迹法测定结合至5 '侧翼区的DNA结合因子的位置。在Adh 1中，有两种类型的DNA结合因子与启动子区相关。一组因素是组成性相关的顺式调节厌氧反应元件，而两个额外的因素结合后，Adh 1已诱导缺氧应激。将缺氧应激的细胞返回到有氧环境中可以恢复未诱导的Adh 1基因观察到的硫酸二甲酯足迹。相反，与Adh 2的5 '侧翼区结合的所有因子组成型存在并且不受缺氧影响。Adh 1和Adh 2有一个共同的足迹位点，但它不是厌氧反应样元素。
The transcriptional activation of maize alcohol dehydrogenase-1 (Adh1) and alcohol dehydrogenase-2 (Adh2) is accompanied by changes in the chromatin structure within the 5'-flanking region of each gene. The positions of DNA-binding factors bound to the 5'-flanking regions were determined by in vivo dimethyl sulfate footprinting of maize suspension cultures over 8 hours of hypoxic induction. In Adh1 there are two types of DNA-binding factors associated with the promoter region. One set of factors is constitutively associated with the cis-regulatory anaerobic response element, whereas two additional factors bind only after Adh1 has been induced by hypoxic stress. Returning hypoxically stressed cells to an aerobic environment restores the dimethyl sulfate footprint observed for the uninduced Adh1 gene. In contrast, all of the factors bound to the 5'-flanking region of Adh2 are constitutively present and unchanged by hypoxia. There is one footprint site common to both Adh1 and Adh2, but it is not an anaerobic response-like element.