Induction of bone formation in biphasic calcium phosphate scaffolds by bone morphogenetic protein‐2 and primary osteoblasts
Induction of bone formation in biphasic calcium phosphate scaffolds by bone morphogenetic protein‐2 and primary osteoblasts
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DOI：
10.1002/term.1511
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发表时间：
2014-03
影响因子：
3.3
通讯作者：
L. Strobel;S. Rath;Anne-Kathrin Maier;Justus P. Beier;Andreas Arkudas;Peter Greil;R. Horch;Ulrich Kneser
中科院分区：
文献类型：
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作者：
L. Strobel;S. Rath;Anne-Kathrin Maier;Justus P. Beier;Andreas Arkudas;Peter Greil;R. Horch;Ulrich Kneser
骨组织工程的策略主要依赖于多孔支架材料。在这项研究中，通过3D打印生成了新型双相磷酸钙（BCP）基质。通过淀粉固结实现高孔隙率。本研究旨在验证多孔BCP-支架的特性以及在体内条件下成骨细胞和生长因子的相互作用。将5种不同处理的构建体皮下植入同系大鼠体内：普通BCP构建体（A组）、经BMP-2预处理的构建体（B组;每个支架1.6 µg BMP-2）、接种原代成骨细胞（OB）（C组）、接种OB和BMP-2（D组）以及接种OB并在流动生物反应器中预培养6周的构建体（E组）。分别于术后2、4、6周取材，进行组织学和分子生物学分析。取出的支架被纤维血管组织侵入，没有明显的异物反应。形态测定分析表明，与所有其他组相比，D组（OB + BMP-2）样本的骨形成显著增加。6周后，来自B-E组的样品显示骨特异性基因的显著mRNA表达。在流动生物反应器（E组）中预培养诱导的骨形成与B组相当。在这项研究中，可以观察到BMP-2或成骨细胞样本之间的骨分布差异。总之，成骨细胞和BMP-2的组合协同增强了新型陶瓷支架中的骨形成。这些结果提供了进一步的原位缺损模型的实验基础，重点是在骨科和重建手术的未来应用。版权所有© 2012约翰威利父子有限公司.
Bone tissue engineering strategies mainly depend on porous scaffold materials. In this study, novel biphasic calcium phosphate (BCP) matrices were generated by 3D‐printing. High porosity was achieved by starch consolidation. This study aimed to characterise the porous BCP‐scaffold properties and interactions of osteogenic cells and growth factors under in vivo conditions. Five differently treated constructs were implanted subcutaneously in syngeneic rats: plain BCP constructs (group A), constructs pre‐treated with BMP‐2 (group B; 1.6 µg BMP‐2 per scaffold), seeded with primary osteoblasts (OB) (group C), seeded with OB and BMP‐2 (group D) and constructs seeded with OB and pre‐cultivated in a flow bioreactor for 6 weeks (group E). After 2, 4 and 6 weeks, specimens were explanted and subjected to histological and molecular biological analyses. Explanted scaffolds were invaded by fibrovascular tissue without significant foreign body reactions. Morphometric analysis demonstrated significantly increased bone formation in samples from group D (OB + BMP‐2) compared to all other groups. Samples from groups B‐E displayed significant mRNA expression of bone‐specific genes after 6 weeks. Pre‐cultivation in the flow bioreactor (group E) induced bone formation comparable with group B. In this study, differences in bone distribution between samples with BMP‐2 or osteoblasts could be observed. In conclusion, combination of osteoblasts and BMP‐2 synergistically enhanced bone formation in novel ceramic scaffolds. These results provide the basis for further experiments in orthotopic defect models with a focus on future applications in orthopaedic and reconstructive surgery. Copyright © 2012 John Wiley & Sons, Ltd.