CORRELATION BETWEEN SALIVARY AND SERUM MELATONIN - DEPENDENCE ON SERUM MELATONIN LEVELS
CORRELATION BETWEEN SALIVARY AND SERUM MELATONIN - DEPENDENCE ON SERUM MELATONIN LEVELS
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DOI：
10.1111/j.1600-079x.1990.tb00692.x
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发表时间：
1990-01-01
影响因子：
10.3
通讯作者：
JOHANSSON, G
中科院分区：
文献类型：
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作者：
LAAKSO, ML;PORKKAHEISKANEN, T;JOHANSSON, G
在26小时的时间里，8名健康志愿者每两小时收集一次唾液和血清样本。用放射性碘标记的褪黑素作为示踪剂，用放射免疫法测定氯仿提取后的褪黑素浓度。其中5名受试者夜间血清褪黑素水平较高(峰值高于75pg/ml)，3名受试者血清褪黑素最高浓度为20-40pg/ml，所有受试者白天血清褪黑素水平均较低(<10pg/ml)。计算唾液和血清浓度之间的相关性。所有可测值对(n=73)的回归方程为y=0.33x+3.7pg/ml，r=0.95P<；0.001。褪黑素的峰值和分泌模式的上升期和下降期的回归系数和相关系数几乎相等。然而，单独计算时，白天唾液浓度低和血清浓度之间没有明显的相关性。在5个高分泌组中，唾液中的褪黑素水平较可靠地反映了血清的变化，但在3个低分泌组中，唾液与血清褪黑素的相关性不显著。唾液中发现的褪黑素比例随着血清褪黑素水平的增加而减少。昼夜节律参数的估计采用单因素方差分析。在血清和唾液褪黑激素的伴随测量中，受试者的顶峰时相没有显著差异。唾液褪黑素水平的测量似乎对褪黑素节律的研究是有效的，但唾液中测量的褪黑素浓度并不总是一致地反映血液中的绝对浓度。
Saliva and serum samples were collected from eight healthy volunteers every two hours during a 26-hour period. Melatonin concentrations were measured by radioimmunoassay after chloroform extraction using radioiodinated melatonin as a tracer. Five of the subjects had high serum melatonin levels at night (peak levels higher than 75 pg/ml); in three subjects the highest serum melatonin concentration was 20-40 pg/ml. All subjects had low levels (< 10 pg/ml) during the day. The correlations between salivary and serum levels were calculated. The regression line y = 0.33x + 3.7 pg/ml, r = 0.95, P < 0.001, was obtained for all detectable value pairs (n = 73). The regression and correlation coefficients were almost equal for the peak values of melatonin and during the rising and descending phases of the secretion patterns. However, no significant correlation was found between low daytime salivary and serum concentrations when calculated separately. In the five high-secretors the melatonin levels in saliva reflected reliably the changes in serum, but in the three low-secretors the correlation between salivary and serum melatonin was not significant. The proportion of melatonin found in saliva decreased with increasing serum melatonin levels. Circadian rhythm parameters were estimated by single cosinor analysis. The acrophases did not differ significantly within a subject in the concomitant measurements of serum and salivary melatonin. The measurements of salivary melatonin levels seem valid for studies on melatonin rhythms, but the melatonin concentrations measured in saliva do not always consistently reflect the absolute concentrations in blood.