Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast
Widespread use of TATA elements in the core promoters for RNA polymerases III, II, and I in fission yeast
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DOI：
10.1128/mcb.21.20.6870-6881.2001
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发表时间：
2001-10-01
影响因子：
5.3
通讯作者：
Maraia, RJ
中科院分区：
文献类型：
--
作者：
Hamada, M;Huang, Y;Maraia, RJ
除了指导转录起始之外，核心启动子整合来自远端调控元件的输入。除了极少数例外，一般发现真核tRNA和rRNA基因不含TATA启动子元件，而是利用蛋白质-蛋白质相互作用将TATA结合蛋白（TBP）带到核心启动子。全基因组分析揭示了TATA元件在粟酒裂殖酵母中tRNA和5S rRNA（Pol III）、U1至U 5 snRNA（Pol II）和37 S rRNA（Pol I）基因的核心启动子中。使用tRNA依赖性抑制和其他体内试验，以及体外转录，我们证明了上游TATA元件的tRNA和5S rRNA在S.粟球。在S. pombe，而TBP不是，与TATA独立招聘TBP一致。模板定型分析与此一致，并证实了转录复合物组装和启动的机制，在S。粟酒裂殖酵母与其他模式生物的差异很大。结果被扩展到大rRNA合成，因为Pol I启动子中的TATA元件的突变也废除了裂殖酵母中的rRNA表达。对其他生物基因组的调查显示，大量的真核生物可能使用广泛的TATA进行转录。这些结果表明，TATA统一的转录系统在当代真核生物的存在下，并提供洞察TBP的剩余需要在其他真核生物中的所有三个Pol，尽管缺乏TATA元件在其启动子。
In addition to directing transcription initiation, core promoters integrate input from distal regulatory elements. Except for rare exceptions, it has been generally found that eukaryotic tRNA and rRNA genes do not contain TATA promoter elements and instead use protein-protein interactions to bring the TATA-binding protein (TBP), to the core promoter. Genomewide analysis revealed TATA elements in the core promoters of tRNA and 5S rRNA (Pol III), U1 to U5 snRNA (Pol II), and 37S rRNA (Pol I) genes in Schizosaccharomyces pombe. Using tRNA-dependent suppression and other in vivo assays, as well as in vitro transcription, we demonstrated an obligatory requirement for upstream TATA elements for tRNA and 5S rRNA expression in S. pombe. The Pol III initiation factor Brf is found in complexes with TFIIIC and Pol III in S. pombe, while TBP is not, consistent with independent recruitment of TBP by TATA. Template commitment assays are consistent with this and confirm that the mechanisms of transcription complex assembly and initiation by Pol III in S. pombe differ substantially from those in other model organisms. The results were extended to large-rRNA synthesis, as mutation of the TATA element in the Pol I promoter also abolishes rRNA expression in fission yeast. A survey of other organisms' genomes reveals that a substantial number of eukaryotes may use,widespread TATAs for transcription. These results indicate the presence of TATA-unified transcription systems in contemporary eukaryotes and provide insight into the residual need for TBP by all three Pols in other eukaryotes despite a lack of TATA elements in their promoters.