The role of genotype and diet in shaping gut microbiome in a genetic vitamin A deficient mouse model
The role of genotype and diet in shaping gut microbiome in a genetic vitamin A deficient mouse model
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基因型和饮食在遗传性维生素 A 缺乏小鼠模型中肠道微生物群塑造中的作用
DOI：
10.1016/j.jgg.2021.08.015
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发表时间：
2022-02-28
影响因子：
5.9
通讯作者：
Chen, Feng
中科院分区：
文献类型：
--
作者：
Xu, Jun;Zhang, Jie-Ni;Chen, Feng
据报道，多种因素会影响肠道微生物组，包括基因型、年龄、饮食和营养。然而，很少有报道在一项研究中研究不同因素对肠道微生物组形成的相对能力。我们的设计使用遗传性维生素A缺乏小鼠模型，Rbp 4（-/-）小鼠，在不同的起始年龄（4或7周）用低维生素A饮食喂养28天。在饮食控制后的七个时间点收集粪便样品用于细菌谱分析。随着Rbp 4的消耗，阿克曼氏菌减少，拟杆菌增加，而脱硫弧菌，巴梅氏菌，梭菌XIVa和乳酸杆菌波动。细菌群落随着维生素A缺乏饮食的施用而迅速调整并逐渐改变（例如，减少Bamesiella和增加脱硫弧菌）。年龄的影响相对较弱，但持续时间较长。在较早的年龄喂养维生素A缺乏的饮食，更高的微生物生态失调指数将受到重视。值得注意的是，饮食和年龄对细菌群落的塑造作用随基因型的差异而变化，这可能表明基因型在塑造肠道微生物组方面的作用大于饮食和年龄。中国科学院遗传与发育生物学研究所版权所有（C）2021由爱思唯尔有限公司和科学出版社出版。All rights reserved.
Multifactors have been reported to affect the gut microbiome, including genotype, age, diet, and nutrition. However, few reports have investigated the relative capacity of different factors to shape the gut micro-biome in a single study. Our design used a genetic vitamin A-deficient mouse model, the Rbp4(-/-) mouse, feeding with the low vitamin A diets at different ages of initiation (4 or 7 weeks) for 28 days. Fecal samples were collected for bacterial profiling at seven time points after diet controlling. With Rbp4 depletion, Akkermansia decreased and Bacteroides increased, whereas Desulfovibrio, Bamesiella, Clostridium_XIVa, and Lactobacillus fluctuated. The bacterial community swiftly adjusted with the vitamin A-deficient diet administration and gradually changed (e.g., decrease of Bamesiella and increase of Desulfovibrio). Age exerted a relatively weaker but long-last influence. At an earlier age to feed a vitamin A-deficient diet, a higher microbial dysbiosis index will be valued. Of note, the shaping effects of diet and age on the bacterial community varied with the difference of genotype, which might indicate a greater role of genotype than diet and age in shaping the gut microbiome. Copyright (C) 2021, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.