A murine fibroblast growth factor (FGF) receptor expressed in CHO cells is activated by basic FGF and Kaposi FGF.
A murine fibroblast growth factor (FGF) receptor expressed in CHO cells is activated by basic FGF and Kaposi FGF.
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DOI：
10.1073/pnas.87.11.4378
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发表时间：
1990-06
影响因子：
11.1
通讯作者：
A. Mansukhani;D. Moscatelli;D. Talarico;V. Levytska;C. Basilico
中科院分区：
文献类型：
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作者：
A. Mansukhani;D. Moscatelli;D. Talarico;V. Levytska;C. Basilico
我们克隆了一个小鼠互补DNA（cDNA），它编码一种酪氨酸激酶受体，与鸡成纤维细胞生长因子（FGF）受体和人fms样基因（FLG）酪氨酸激酶约有90%的相似性。这种小鼠受体在细胞外部分缺失88个氨基酸，与鸡FGF受体的三个免疫球蛋白样结构域相比，仅剩下两个。该cDNA被克隆到一个表达载体中，并转染到受体阴性的中国仓鼠卵巢（CHO）细胞中。我们表明，表达该受体的细胞能够结合碱性FGF和卡波西FGF。尽管该受体与碱性FGF的亲和力高15到20倍，但卡波西FGF能够诱导受体下调的程度与碱性FGF相同。在两种FGF刺激下，受体都会被磷酸化，DNA合成被刺激，并且在表达该受体的细胞中产生增殖反应，而以反义方向表达该cDNA的细胞对碱性FGF或卡波西FGF没有这些反应。因此，这种受体能够与FGF家族的两种生长因子发生功能性相互作用。
We have cloned a murine cDNA encoding a tyrosine kinase receptor with about 90% similarity to the chicken fibroblast growth factor (FGF) receptor and the human fms-like gene (FLG) tyrosine kinase. This mouse receptor lacks 88 amino acids in the extracellular portion, leaving only two immunoglobulin-like domains compared to three in the chicken FGF receptor. The cDNA was cloned into an expression vector and transfected into receptor-negative CHO cells. We show that cells expressing the receptor can bind both basic FGF and Kaposi FGF. Although the receptor binds basic FGF with a 15- to 20-fold higher affinity, Kaposi FGF is able to induce down-regulation of the receptor to the same extent as basic FGF. The receptor is phosphorylated upon stimulation with both FGFs, DNA synthesis is stimulated, and a proliferative response is produced in cells expressing the receptor, whereas cells expressing the cDNA in the antisense orientation show none of these responses to basic FGF or Kaposi FGF. Thus this receptor can functionally interact with two growth factors of the FGF family.