Isolation and identification of 23,25-dihydroxyvitamin D3, an in vivo metabolite of vitamin D3.
Isolation and identification of 23,25-dihydroxyvitamin D3, an in vivo metabolite of vitamin D3.
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23,25-二羟基维生素 D3（维生素 D3 的体内代谢物）的分离和鉴定。
DOI：
10.1021/bi00516a032
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Tanaka,Y;Wichmann,JK;Schnoes,HK;DeLuca,HF
田中洋子、约瑟夫·K.作者声明：Heinrich K. Schnoes和Hector F. DeLuca* 摘要：补充维生素D的大鼠产生25-羟基[3a-3 H]维生素D3的代谢物，其易于通过使用高效液相色谱法与已知代谢物分离。这种代谢物在体内的产生以及1，25-二羟维生素D3，24（J？），25-二羟维生素D3和25-羟维生素D3 26，23-内酯通过肾切除术被大部分（如果不是全部）消除。补充维生素D的鸡的肾匀浆与25-羟基维生素D3孵育产生大量的新的未知代谢产物。通过使用直相和反相色谱法从这种孵育混合物中以纯的形式分离该代谢物。
Yoko Tanaka, Joseph K. Wichmann, Heinrich K. Schnoes, and Hector F. DeLuca* abstract: Vitamin D supplemented rats produce a metabolite of 25-hydroxy [3a-3H] vitamin D3 that is easily separated from known metabolites by using high-performance liquid chromatography. The production of this metabolite in vivo as well as 1, 25-dihydroxyvitamin D3, 24 (J?), 25-dihydroxyvitamin D3, and 25-hydroxyvitamin D3 26, 23-lactone is largely if not totally eliminated by nephrectomy. Kidney homogenates from vitamin D supplemented chickens incubated with 25-hydroxyvitamin D3 produce significant quantities of the new, unknown metabolite. This metabolite was isolated in pure form from such incubation mixtures by using both straight-phase and