Antimicrobial activity of Lactobacillus fermentum TcUESCO1 against Streptococcus mutans UA159
Antimicrobial activity of Lactobacillus fermentum TcUESCO1 against Streptococcus mutans UA159
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DOI：
10.1016/j.micpath.2020.104063
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发表时间：
2020-05-01
影响因子：
3.8
通讯作者：
Yatsuda, Regiane
中科院分区：
文献类型：
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作者：
de Souza Rodrigues, Jeisa Zielle;Passos, Manuela Ribeiro;Yatsuda, Regiane
龋齿是一种多因素慢性感染性疾病，其开始于主要由变形链球菌引起的细菌生物膜形成。益生菌的使用已经显示出许多健康益处，包括在对抗口腔疾病方面。发酵乳杆菌菌株已经显示出对S.变异人，但研究仍然很少。因此，我们研究的目的是评价接种物和代谢产物的抗菌活性的L。Fermentum TcUESC 01对S.变异UA 159.为此，建立了L.进行发酵，并测试接种物和发酵中形成的代谢物对S.在琼脂扩散试验中，仅测试其代谢产物以确定最小抑制浓度、最小杀菌浓度和细胞粘附抑制。还用代谢物测试了生物膜形成、pH下降和质子渗透性的抑制。抑菌圈在14 h开始形成，并持续至16 h。接种L.发酵菌也显示出抑制区。代谢产物的MIC为1280 mg/mL，获得的MBC浓度高于等于5120 mg/mL的MIC。抑制S.变形菌粘附到微孔板的表面。在生物膜分析中，与对照生物膜相比，用测试浓度的代谢物处理不能减少生物量、不溶性葡聚糖和碱溶性葡聚糖（p > 0.05）。代谢产物对S.与生理盐水组相比，生物膜组中的变形细胞数明显减少（P > 0.05）。乳酸（50.38%）是L.发酵菌这是第一份报告表明发酵乳杆菌TcUESCO 1产生的代谢产物具有用作抗S.变形链球菌，显示出抗粘附和杀菌活性，对嗜酸性细胞的S。变种人因此，为了更好地了解L.发酵菌TCUESC 01。
Dental caries is a multifactorial chronic-infection disease, which starts with a bacterial biofilm formation caused mainly by Streptococcus mutans. The use of probiotics has shown numerous health benefits, including in the fight against oral diseases. Strains of Lactobacillus fermentum have already shown probiotic potential against S. mutans, but there are still few studies. Thus, the aim of our study was to evaluate the antimicrobial activity of the inoculum and metabolites produced by L. fermentum TcUESC01 against S. mutans UA159. For this, a growth curve of L. fermentum was performed and both the inoculum and the metabolites formed in the fermentation were tested against the growth of S. mutans UA159 in agar diffusion tests, and only its metabolites were tested to determine the minimum inhibitory concentration, minimal bactericidal concentration and inhibition of cell adhesion. Inhibition of biofilm formation, pH drop and proton permeability were also tested with the metabolites. The zone of inhibition began to be formed at 14 h and continued until 16 h. The inoculum containing L. fermentum also showed zone of inhibition. The MIC for the metabolites was 1280 mg/mL and the MBC was obtained with a concentration higher than the MIC equal to 5120 mg/mL. Half of the MIC concentration (640 mg/mL) was required to inhibit S. mutans adhesion to the surface of the microplates. In the biofilm analyzes, the treatment with the metabolites in the tested concentration was not able to reduce biomass, insoluble glucans and alkali soluble compared to the control biofilm (p > 0.05). The metabolites also did not affect acid production and acid tolerance of S. mutans cells in biofilms compared to saline group (p > 0.05). Lactic acid (50.38%) was the most abundant organic acid produced by L. fermentum. This is the first report showing that the metabolites produced by the Lactobacillus fermentum TcUESCO1 have a potential to be used as an antimicrobial agent against S. mutans, showing anti-adherence and bactericidal activity against planktonic cells of S. mutans. Thus, further studies should be carried out in order to better understand the antimicrobial activity of metabolites of L. fermentum TCUESC01.