Identification of proteins interacting with GTP cyclohydrolase I.
Identification of proteins interacting with GTP cyclohydrolase I.
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DOI：
10.1016/j.bbrc.2009.05.026
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发表时间：
2009-07-24
影响因子：
3.1
通讯作者：
Shi, Yang
中科院分区：
文献类型：
--
作者：
Du, Jianhai;Xu, Hao;Wei, Na;Wakim, Bassam;Halligan, Brian;Pritchard, Kirkwood A., Jr.;Shi, Yang
关键词：
GTP cyclohydrolase I (GCH-1) is the rate-limiting enzyme in the biosynthesis of tetrahydrobiopterin, an essential cofactor for nitric oxide synthase and aromatic amino acid hydroxylase. To explore the interactome of GCH-1, we established a HEK293 cell line stably expressing tetracycline-inducible FLAG-GCH-1. FLAG-GCH-1 and associated proteins were immunoprecipitated and analyzed by liquid chromatography/tandem mass spectrometry. Twenty-nine proteins, derived from different subcellular components such as cytosol, membranes, nucleus and mitochondria were identified to interact with GCH-1. Cell fractionation studies also showed that GCH-1 was present in the cytosol, membranes and nucleus. Gene ontology analysis revealed that GCH-1 interactome was involved in a variety of biological processes such as signal transduction, apoptosis, metabolism, transport and cell organization. To our knowledge, this study is the first to provide a comprehensive analysis of the GCH-1 interactome. Findings expand the number and diversity of proteins that are known to associate with GCH-1.
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