The osteoclastogenic molecules RANKL and RANK are associated with periprosthetic osteolysis
The osteoclastogenic molecules RANKL and RANK are associated with periprosthetic osteolysis
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DOI：
10.1302/0301-620x.83b6.10905
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发表时间：
2001-08-01
影响因子：
--
通讯作者：
Findlay, DM
中科院分区：
文献类型：
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作者：
Haynes, DR;Crotti, TN;Findlay, DM
种植体附近广泛的骨溶解通常与假体材料的磨损颗粒有关。我们已经研究了RANKL，也被称为骨保护素配体，破骨细胞分化因子或TRANCE，及其天然抑制剂骨保护素（OPG）是否在控制这种骨质流失中起重要作用。从含有磨损颗粒的假体周围组织中分离的细胞表达编码破骨促生分子、RANKL及其受体RANK、单核细胞集落刺激因子（M-CSF）、白细胞介素(IL)-1 β、肿瘤坏死因子(TNT) α、IL-6和可溶性IL-6受体以及OPG的mRNA。在人成骨细胞存在和不存在的情况下，从假体周围组织分离的细胞形成破骨细胞。当在成骨细胞缺失的情况下形成破骨细胞时，RANKL mRNA的表达水平明显高于OPG mRNA。假体材料颗粒在体外也能刺激人单核细胞表达破骨细胞分子。我们的研究结果表明，巨噬细胞摄取假体磨损颗粒导致破骨分化分子的表达，并刺激巨噬细胞分化为破骨细胞。
Extensive osteolysis adjacent to implants is often associated with wear particles of prosthetic material. We have investigated if RANKL, also known as osteoprotegerin ligand, osteoclast differentiation factor or TRANCE, and its natural inhibitor, osteoprotegerin (OPG), may be important in controlling this bone loss.Cells isolated from periprosthetic tissues containing wear particles expressed mRNA encoding for the pro-osteoclastogenic molecules, RANKL, its receptor RANK, monocyte colony-stimulating factor (M-CSF), interleukin (IL)-1 beta, tumour necrosis factor (TNT)alpha, IL-6, and soluble IL-6 receptor, as well as OPG. Osteoclasts formed from cells isolated from periprosthetic tissues in the presence and absence of human osteoblastic cells. When osteoclasts formed in the absence of osteoblastic cells, markedly higher levels of RANKL mRNA relative to OPG mRNA were expressed. Particles of prosthetic materials also stimulated human monocytes to express osteoclastogenic molecules in vitro.Our results suggest that ingestion of prosthetic wear particles by macrophages results in expression of osteoclast-differentiating molecules and the stimulation of macrophage differentiation into osteoclasts.