Hearing Improvement in A/J Mice via the Mouse Nerve Growth Factor.
Hearing Improvement in A/J Mice via the Mouse Nerve Growth Factor.
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DOI：
10.21053/ceo.2016.01354
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发表时间：
2017-12
影响因子：
3
通讯作者：
Han F
中科院分区：
文献类型：
--
作者：
Gao L;Ge R;Xie G;Yao D;Li P;Wang O;Ma X;Han F
目的探讨小鼠神经生长因子（mNGF）对A/J小鼠耳的保护作用。 将出生后第7天（P7）的小鼠随机分为mNGF治疗组（mNGF组）和蒸馏水（注射用）治疗组（对照组）。从P7开始，通过臀部肌内注射，将溶解在蒸馏水中的mNGF或仅蒸馏水给予小鼠，每隔一天一次。以时间过程的方式观察mNGF对A/J小鼠耳的保护作用。于3 ~ 8周龄测定脑干诱发电位（ABR）阈值。分别于3、6、8周龄取内耳切片，HE染色，观察螺旋神经节神经元（SGN）的形态。在8周龄时，对耳蜗的整片外毛细胞（OHC）进行计数。采用实时荧光定量PCR和Western blotting检测凋亡相关基因的表达。 mNGF组ABR阈值在6、8周龄均显著低于对照组。此外，mNGF在此期间保留了小鼠耳蜗中的OHC和SGN。进一步的实验显示mNGF组小鼠内耳caspase基因（包括caspase-3）的表达受到抑制。 mNGF通过保留耳蜗中的SGN和OHC来改善A/J小鼠的听力。
To investigate the otoprotective effects of mouse nerve growth factor (mNGF) in A/J mice. The mice at postnatal day 7 (P7) were randomly separated into a mNGF treated group (mNGF group) and a distilled water (for injection) treated group (control group). The mNGF dissolved in distilled water or distilled water alone was given to the mice once every other day from P7 by intramuscular injection in the hips. The otoprotective effects of mNGF in A/J mice were observed in a time course manner. The thresholds of auditory-evoked brainstem response (ABR) were tested from the age of the 3rd to the 8th week. Sections of the inner ears were stained by hematoxylin and eosin, and spiral ganglion neurons (SGNs) were observed at the age of the 3rd, the 6th,and the 8th week. Counts of whole mount outer hair cells (OHCs) in the cochleae were made at the age of 8 weeks. Expression of apoptosis related genes was determined by quantitative real-time polymerase chain reaction and Western blotting. ABR thresholds of the mNGF group were significantly lower than those of the control group at the age of the 6th and the 8th week. Moreover, the mNGF preserved OHC and SGN in the mouse cochleae in this period. Further experiments showed that the expression of caspase genes (including caspase-3) was inhibited in the mouse inner ears in the mNGF group. The mNGF improves hearing in A/J mice by preserving SGN and OHC in the cochleae.