Closed nutrient recycling via microbial catabolism in an eco-engineered self regenerating mixed anaerobic microbiome for hydrogenotrophic methanogenesis.
Closed nutrient recycling via microbial catabolism in an eco-engineered self regenerating mixed anaerobic microbiome for hydrogenotrophic methanogenesis.
复制标题
DOI：
10.1016/j.biortech.2016.12.052
复制
发表时间：
2017-03
影响因子：
11.4
通讯作者：
Savvas Savvas-Savvas;J. Donnelly;Tim Patterson;R. Dinsdale;S. Esteves
中科院分区：
文献类型：
--
作者：
Savvas Savvas-Savvas;J. Donnelly;Tim Patterson;R. Dinsdale;S. Esteves
一种新型的生态工程混合厌氧培养物首次被成功地证明能够在营养限制条件下连续再生。已发现微生物催化剂支持封闭的营养物系统，其能够富集嗜石产甲烷菌的培养物并提供微生物细胞再循环。富集后，氢营养物种是占主导地位的产甲烷菌，而细菌基质是负责营养物质的再分配。q-PCR结果表明，总种群的7%负责气体的直接转化。当产气速率大于60 v/v/d时，H2/CO2转化为CH 4的效率可达100%。通过由溶解的CO2产生的缓冲系统，培养基的pH有效地维持在最佳水平（pH 7-8）。该新方法可以降低过程营养物/金属需求，并提高用于可再生能源储存的氢营养产甲烷的环境和经济性能。
A novel eco-engineered mixed anaerobic culture was successfully demonstrated for the first time to be capable of continuous regeneration in nutrient limiting conditions. Microbial catabolism has been found to support a closed system of nutrients able to enrich a culture of lithotrophic methanogens and provide microbial cell recycling. After enrichment, the hydrogenotrophic species was the dominating methanogens while a bacterial substratum was responsible for the redistribution of nutrients. q-PCR results indicated that 7% of the total population was responsible for the direct conversion of the gases. The efficiency of H2/CO2conversion to CH4reached 100% at a gassing rate of above 60 v/v/d. The pH of the culture media was effectively sustained at optimal levels (pH 7–8) through a buffering system created by the dissolved CO2. The novel approach can reduce the process nutrient/metal requirement and enhance the environmental and financial performance of hydrogenotrophic methanogenesis for renewable energy storage.