RHODOPSIN AND RETINOBLASTOMA - A MONOCLONAL-ANTIBODY HISTOPATHOLOGIC STUDY
RHODOPSIN AND RETINOBLASTOMA - A MONOCLONAL-ANTIBODY HISTOPATHOLOGIC STUDY
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DOI：
10.1001/archopht.1986.01050130125035
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发表时间：
1986-01-01
影响因子：
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通讯作者：
ARBIZO, V
中科院分区：
文献类型：
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作者：
DONOSO, LA;HAMM, H;ARBIZO, V
使用单克隆抗体（MAb） MAb- e在甲醛固定、石蜡包埋的人胎儿视网膜和5个视网膜母细胞瘤中鉴定了视紫红质。然后用另一种单克隆抗体MAbA9-C6与s抗原比较视紫红质免疫反应性对应的结合模式。视紫红质和s抗原首次在18周大的人胎眼中观察到，处于光感受器分化之前的阶段。在含有正常感光细胞的成人眼睛中，视紫红质免疫反应仅限于视杆外段，而s抗原免疫反应则局限于整个感光细胞。在视网膜母细胞瘤中，这两种单克隆抗体结合到肿瘤的同一区域；然而，每个单克隆抗体都有不同的染色模式。在4例病例中，与视紫红质相关的细胞表面结合特征是强烈的、界限明确的“晕”模式，而与s抗原相关的结合模式在所有病例中都是强烈的、定位良好的、细胞质性的。我们的研究结果表明，一些分化良好的视网膜母细胞瘤同时表达视紫红质和s抗原，并因此表达参与视觉光转导初始事件的蛋白质。
Rhodopsin was identified in formaldehyde-fixed, paraffin-embedded human fetal retina, and in five retinoblastomas using monoclonal antibody (MAb) MAb-E. The binding pattern corresponding to rhodopsin immunoreactivity was then compared with S-antigen using another monoclonal antibody, MAbA9-C6. Rhodopsin and S-antigen were first observed in the 18-week-old human fetal eye, at a stage preceding photoreceptor differentiation. In adult eyes containing normal photoreceptor cells, rhodopsin immunoreactivity was restricted to the rod outer segments, whereas S-antigen immunoreactivity was localized to the entire photoreceptor cell. In retinoblastomas both monoclonal antibodies bound to the same area of the tumor; however, different and distinct staining patterns associated with each monoclonal antibody were recognized. In four cases, an intense well-circumscribed "halo" pattern, characteristic of cell-surface binding, was associated with rhodopsin, whereas the binding pattern associated with S-antigen was intense, well localized, and cytoplasmic in all cases. Our results show that some well-differentiated retinoblastomas express both rhodopsin and S-antigen, and as such express proteins that participate in the initial events in the phototransduction of vision.