Genome editing reveals the function of Yorkie during the embryonic and early larval development in silkworm, Bombyx mori
Genome editing reveals the function of Yorkie during the embryonic and early larval development in silkworm, Bombyx mori
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基因组编辑揭示了 Yorkie 在蚕胚胎和早期幼虫发育过程中的功能
DOI：
10.1111/imb.12502
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发表时间：
2018-08
影响因子：
2.6
通讯作者：
X. G Zhou
中科院分区：
文献类型：
--
作者：
X Xu;Z Zhang;Y Yang;S Huang;K Li;L. He;X. G Zhou
Yorkie（Yki）是Hippo信号通路的下游靶点，作为转录辅激活因子，在动物发育和再生过程中调节器官大小。先前对家蚕的微阵列分析表明，与Hippo通路相关的基因主要在性腺和成虫盘中表达。RNA干扰介导的Yki在早期游走阶段的沉默延迟了B。家蚕发育和卵巢成熟，而杆状病毒介导的过表达在幼虫后期促进器官生长和加速变态。在这里，我们采用CRISPR/Cas9介导的诱变来研究Yki在B中的功能。桑（BmYki）的胚胎和早期幼虫阶段。敲除BmYki导致体型缩小，蜕皮缺陷，最终导致幼虫死亡。CRISPR/Cas9突变体的序列分析显示BmYki中的一系列缺失。作为Hippo激酶盒的关键下游效应子，BmYki在胚胎阶段的沉默是必不可少的，并且结果是致命的。鉴于Hippo信号通路在进化上是保守的，Yki有可能成为基于遗传的害虫管理实践的新型分子靶点。
As a transcriptional coactivator, Yorkie (Yki) is a major downstream target of the Hippo signalling pathway to regulate the organ size during animal development and regeneration. Previous microarray analysis in the silkworm, Bombyx mori, has shown that genes associated with the Hippo pathway were primarily expressed in gonads and imaginal discs. The RNA‐interference‐mediated silencing of Yki at the early wandering stage delayed B. mori development and ovary maturation, whereas baculovirus‐mediated overexpression at the late larval instar facilitated organ growth and accelerated metamorphosis. Here, we employed CRISPR/Cas9‐mediated mutagenesis to investigate the function of Yki in B. mori (BmYki) at the embryonic and early larval stages. Knocking out of BmYki led to reduced body size, moulting defects and, eventually, larval lethality. Sequence analysis of CRISPR/Cas9 mutants exhibited an array of deletions in BmYki. As a critical downstream effector of the Hippo kinase cassette, silencing of BmYki at the embryonic stage is indispensable and the consequence is lethal. Given that the Hippo signalling pathway is evolutionarily conserved, Yki has the potential to be a novel molecular target for genetic‐based pest management practices.
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影响因子：
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作者：
Dey NS;Ramesh P;Chugh M;Mandal S;Mandal L
通讯作者：
Mandal L
影响因子：
3.3
作者：
Lin, Tzu-Huai;Yeh, Tsung-Han;Yu, Jenn-Yah
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影响因子：
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作者：
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11.8
作者：
Neto-Silva, Ricardo M.;de Beco, Simon;Johnston, Laura A.
通讯作者：
Johnston, Laura A.
DOI：
--
发表时间：
2013
期刊：
--
影响因子：
--
作者：
Woong Y. Hwang;Yanfang Fu;D. Reyon;Morgan L. Maeder;S. Tsai;Jeffry D. Sander;R. Peterson;J.-R. Joanna Yeh-J.
通讯作者：
Woong Y. Hwang;Yanfang Fu;D. Reyon;Morgan L. Maeder;S. Tsai;Jeffry D. Sander;R. Peterson;J.-R. Joanna Yeh-J.