Hydrogen Sulfide Promotes Root Organogenesis in Ipomoea batatas, Salix matsudana and Glycine max
Hydrogen Sulfide Promotes Root Organogenesis in Ipomoea batatas, Salix matsudana and Glycine max
复制标题
DOI：
10.1111/j.1744-7909.2009.00885.x
复制
发表时间：
2009-12-01
影响因子：
11.4
通讯作者：
Hu, Lan-Ying
中科院分区：
文献类型：
--
作者：
Zhang, Hua;Tang, Jun;Hu, Lan-Ying
在这份报告中，我们证明硫氢化钠（NaHS），硫化氢（H2S）供体，促进不定根的形成由生长素和一氧化氮（NO）介导。H_2S供体在甘薯扦插育苗中的应用促进不定根的数量和长度，并呈剂量依赖性。此外，还证实了H2S或HS-而不是来自NaHS的其他含硫组分可以归因于对不定根形成的刺激。HaHS处理后，甘薯茎尖内源H2S、吲哚乙酸（IAA）和NO含量依次迅速增加。进一步的研究表明，IAA转运抑制剂NPA和NO清除剂cPTIO能抑制H2S诱导的根形成。在两种离体杨柳（Salix matsudana var.）中观察到了类似的H2S供体依赖性根器官发生现象。tortuosa Vilm）芽和大豆（Glycine max L.）幼苗这些结果表明，H2S诱导不定根形成的过程可能是由IAA和NO介导的，并且H2S在IAA和NO信号转导途径的上游起作用。
In this report, we demonstrate that sodium hydrosulfide (NaHS), a hydrogen sulfide (H2S) donor, promoted adventitious root formation mediated by auxin and nitric oxide (NO). Application of the H2S donor to seedling cuttings of sweet potato (Ipomoea batatas L.) promoted the number and length of adventitious roots in a dose-dependent manner. It was also verified that H2S or HS- rather than other sulfur-containing components derived from NaHS could be attributed to the stimulation of adventitious root formation. A rapid increase in endogenous H2S, indole acetic acid (IAA) and NO were sequentially observed in shoot tips of sweet potato seedlings treated with HaHS. Further investigation showed that H2S-mediated root formation was alleviated by N-1-naphthylphthalamic acid (NPA), an IAA transport inhibitor, and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), an NO scavenger. Similar phenomena in H2S donor-dependent root organogenesis were observed in both excised willow (Salix matsudana var. tortuosa Vilm) shoots and soybean (Glycine max L.) seedlings. These results indicated that the process of H2S-induced adventitious root formation was likely mediated by IAA and NO, and that H2S acts upstream of IAA and NO signal transduction pathways.