Validation and calibration of DEXA body composition in mice
Validation and calibration of DEXA body composition in mice
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DOI：
10.1152/ajpendo.00470.2002
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发表时间：
2003-09-01
影响因子：
5.1
通讯作者：
Brommage, R
中科院分区：
文献类型：
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作者：
Brommage, R
确定小鼠身体组成的有效方法是研究小鼠肥胖症所必需的。双能X线吸收法（DEXA）提供了一种非侵入性的方法来评估身体脂肪和瘦肉组织的内容。与其他物种的DEXA分析类似，小鼠的体脂测量显示出可接受的精度，但准确性较差。由于脂肪和瘦肉组织各自含有不同的成分，这些不准确性可能是由于选择了不适当的校准标准。溶剂分析表明，PIXImus 2 DEXA给出的结果与理论计算一致。通过PIXImus 2 DEXA和化学尸体分析来分析体重为26-60 g且体脂百分比为3 - 49%的雄性小鼠。DEXA平均高估了小鼠的脂肪含量3.3 g，并生成算法，从测量的体脂值和测量的高能X射线衰减与低能X射线衰减的比率计算体脂。PIXImus 2 DEXA通过对尸体分析测量的小鼠体脂含量进行校准，可提供小鼠准确的身体组成值。
Validated methods of determining murine body composition are required for studies of obesity in mice. Dual-energy X-ray absorptiometry (DEXA) provides a noninvasive approach to assess body fat and lean tissue contents. Similar to DEXA analyses in other species, body fat measurements in mice show acceptable precision but suffer from poor accuracy. Because fat and lean tissues each contain various components, these inaccuracies likely result from selection of inappropriate calibration standards. Analysis of solvents showed that the PIXImus2 DEXA gave results consistent with theoretical calculations. Male mice weighing 26-60 g and having body fat percentages ranging from 3 to 49% were analyzed by both PIXImus2 DEXA and chemical carcass analysis. DEXA overestimated mouse fat content by an average of 3.3 g, and algorithms were generated to calculate body fat from both measured body fat values and the measured ratio of high- to low-energy X-ray attenuations. With calibration to mouse body fat content measured by carcass analysis, the PIXImus2 DEXA gives accurate body composition values in mice.