Sex difference in platelet aggregation detected by new aggregometry using light scattering
Sex difference in platelet aggregation detected by new aggregometry using light scattering
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DOI：
10.1507/endocrj.48.33
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发表时间：
2001-02-01
影响因子：
2
通讯作者：
Makino, T
中科院分区：
文献类型：
--
作者：
Haque, SF;Matsubayashi, H;Makino, T
早期对血小板聚集的研究表明，通过使用光透射（LT）的传统聚集法检测，女性似乎比男性具有更大的聚集性，但争议仍然存在。本研究采用最近发展的激光光散射（LS）方法来确定血小板聚集是否存在性别差异，该方法可以检测小聚集体（即两个或三个血小板）。抽取健康志愿者（10名男性和10名女性，月经后处于卵泡期）的血液，用LT或LS法检测富血小板血浆中的血小板聚集。增加5′-二磷酸腺苷（ADP、0、0.5、1和2 muM）浓度可刺激血小板聚集。为了检测性激素的影响，将血小板与雌二醇（10 nM）或睾酮（40 nM）孵育30分钟，然后进行血小板聚集研究。LT方法显示，雌性比雄性具有更强的聚集性。在弱刺激（小于或等于1 μ m ADP）下，LS法显示雌性比雄性有更多的中等聚集体，睾酮减少小聚集体，雌二醇减少所有大小的聚集体。这些数据表明，在生理ADP浓度（小于或等于1 μ m）下，女性比男性更有利于血小板聚集，但在相同条件下，雌二醇（10 μ m）和睾酮（40 μ m）对血小板聚集都有反作用。因此，女性比男性具有更强的聚集性的部分原因可能是她们缺乏睾丸激素，但其机制仍有待阐明。
Earlier studies in platelet aggregation have shown that females seemed to have greater aggregability than males as detected by conventional aggregometry which used light transmission (LT), but controversy still remains. This study was performed to determine whether sex difference exists in platelet aggregation by using the recently developed laser light scattering (LS) method, which can detect small aggregates (i.e., two or three platelets). Blood was drawn from healthy volunteers (10 male and 10 female in follicular phase after menstruation), and platelet aggregation was detected by either LT or LS method in platelet rich plasma. Platelet aggregation was stimulated by increasing concentration of adenosine 5'-diphosphate (ADP, 0, 0.5, 1 and 2 muM). To detect the effect of sex hormones, platelets were incubated with estradiol (10 nM) or testosterone (40 nM) for 30 min, then platelet aggregation studies were performed. LT method revealed that female had greater aggregability than male. With weak stimuli (less than or equal to 1 muM ADP), LS method showed that females had more medium aggregates than males, and that testosterone decreased small aggregates, and that estradiol decreased all sizes of aggregates. These data suggest that the female is more conductive to platelet aggregation than the male at a physiologic concentration of ADP (less than or equal to 1 muM), but that both estradiol (10 nM) and testosterone (40 nM) have countereffects on platelet aggregation at the same condition. Therefore, the reason why females have greater aggregability than males may partly be explained by their lack of testosterone, but the mechanism still remains to be elucidated.