A highly sensitive enzyme-linked immunosorbent assay for the detection of circulating anti-BP180 autoantibodies in patients with bullous pemphigoid.
A highly sensitive enzyme-linked immunosorbent assay for the detection of circulating anti-BP180 autoantibodies in patients with bullous pemphigoid.
复制标题
一种高灵敏度酶联免疫吸附测定，用于检测大疱性类天疱疮患者的循环抗 BP180 自身抗体。
DOI：
10.1111/1523-1747.ep12338088
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Giudice,GJ
中科院分区：
文献类型：
--
作者：
Zillikens,D;Mascaro,JM;Rose,PA;Liu,Z;Ewing,SM;Caux,F;Hoffmann,RG;Diaz,LA;Giudice,GJ
BP180抗原是表皮锚定复合物的一个组成部分，已被确定为与水泡性皮肤病（大疱性类天疱疮）相关的自身抗体的主要抗原靶点之一。我们的研究小组最近证明，大疱性类天疱疮自身抗体对BP180外结构域的反应性几乎完全局限于聚集在膜近端非胶原拉伸（NC16A）内的一组四个抗原位点。使用被动转移小鼠模型，小鼠BPI80对应的非胶原区域的抗体被证明可以引发炎症性表皮下起泡疾病，这种疾病与大疱性类天疱疮非常相似。我们现在报告了一种酶联免疫吸收测定系统的发展，该系统在检测大疱性类天疱疮患者血清中疾病特异性自身抗体方面非常敏感。该试验的靶抗原是BP180 NC16A结构域的重组形式，该结构域包含所有四个明确的大疱性类天疱疮相关抗原位点。在50个随机选择的大疱性类天疱疮血清中，47个（94%）在该检测中呈阳性，而在107个对照组中没有检测到特异性反应性。有趣的是，所有三种大疱性类天疱疮血清均为阴性，均来自已经接受治疗的患者。NC16A酶联免疫吸附试验比任何检测循环大疱性类天疱疮自身抗体的标准技术更敏感，包括其他酶联免疫吸附试验、免疫印迹和间接免疫荧光。最后，NC16A酶联免疫吸附试验提供了无法从皮肤活检的直接免疫荧光研究中获得的免疫学信息，这可能与大疱性类天疱疮的诊断和治疗有关。
The BP180 antigen, a component of the epidermal anchoring complex, has been identified as one of the major antigenic targets of autoantibodies associated with the blistering skin disease, bullous pemphigoid. Our research group has recently demonstrated that reactivity of bullous pemphigoid autoantibodies to the BP180 ectodomain is almost entirely restricted to a set of four antigenic sites clustered within the membrane-proximal noncollagenous stretch (NC16A). Using a passive transfer mouse model, antibodies to the corresponding noncollagenous region of murine BPI80 were shown to trigger an inflammatory subepidermal blistering disease that closely mimics bullous pemphigoid. We now report the development of an enzyme-linked immunoabsorbent assay system that is extremely sensitive in detecting disease-specific autoantibodies in the sera of bullous pemphigoid patients. The target antigen in this assay is a recombinant form of the BP180 NC16A domain that contains all four of the well-defined bullous pemphigoid-associated antigenic sites. Of 50 randomly selected bullous pemphigoid sera tested, 47 (94%) were positive in this assay, whereas no specific reactivity was detected in any of the 107 controls. Interestingly, all three of the bullous pemphigoid sera that were negative in this assay had been obtained from patients who were already undergoing treatment. The NC16A enzyme-linked immunosorbent assay is more sensitive than any of the standard techniques for detecting circulating bullous pemphigoid autoantibodies, including other enzyme-linked immunosorbent assays, immunoblotting, and indirect immunofluorescence. Finally, the NC16A enzyme-linked immunosorbent assay provides immunologic information that cannot be obtained from direct immunofluorescence studies of skin biopsies, and that may well be relevant in the diagnosis and treatment of bullous pemphigoid.