Inhibition of propofol on single neuron and neuronal ensemble activity in prefrontal cortex of rats during working memory task
Inhibition of propofol on single neuron and neuronal ensemble activity in prefrontal cortex of rats during working memory task
复制标题
异丙酚对大鼠工作记忆任务中前额皮质单个神经元和神经元群活动的抑制作用
DOI：
10.1016/j.bbr.2014.05.034
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发表时间：
2014-08-15
影响因子：
2.7
通讯作者：
Tian, Xin
中科院分区：
文献类型：
--
作者：
Xu, Xinyu;Tian, Yu;Tian, Xin
工作记忆（Working Memory，WM）是指对完成复杂认知任务所需信息的暂时性存储和加工。异丙酚麻醉是否以及如何抑制WM功能越来越受到关注。本研究旨在从单个神经元和神经元整体活动的角度探讨异丙酚麻醉的可能抑制机制。成年SD大鼠随机分为异丙酚组（0.9 mg kg（-1）min（-1），2 h经尾静脉插管）和对照组。在异丙酚麻醉后24、48、72 h，采用Y迷宫奖励交替任务（一种延迟的非匹配样本任务）检测大鼠的工作记忆成绩，同时记录WM任务的行为学结果。从原始信号中获得动作电位的时空序列。单个神经元活动的特点是围事件时间直方图分析和神经元的整体活动的特点是格兰杰因果关系来描述神经元整体内的相互作用。结果表明：与对照组相比，与WM相关的兴奋神经元百分比明显减少（24 h P < 0.01，48 h P < 0.05）;神经元系综内的相互作用明显减弱（24 h p < 0.01，48 h p < 0.05），而72 h无显著性差异（p > 0.05），与行为学结果一致。这些发现有助于从单个神经元活动和神经元整体相互作用的角度进一步理解麻醉抑制WM功能的机制。(C)2014爱思唯尔有限公司版权所有。
Working memory (WM) refers to the temporary storage and manipulation of information necessary for performance of complex cognitive tasks. There is a growing interest in whether and how propofol anesthesia inhibits WM function. The aim of this study is to investigate the possible inhibition mechanism of propofol anesthesia from the view of single neuron and neuronal ensemble activities. Adult SD rats were randomly divided into two groups: propofol group (0.9 mg kg(-1) min(-1), 2 h via a tail vein catheter) and control group. All the rats were tested for working memory performances in a Y-maze-rewarded alternation task (a task of delayed non-matched-to-sample) at 24, 48, 72 h after propofol anesthesia, and the behavior results of WM tasks were recorded at the same time. Spatio-temporal trains of action potentials were obtained from the original signals. Single neuron activity was characterized by peri-event time histograms analysis and neuron ensemble activities were characterized by Granger causality to describe the interactions within the neuron ensemble. The results show that: comparing with the control group, the percentage of neurons excited and related to WM was significantly decreased (p < 0.01 in 24 h,p < 0.05 in 48 h); the interactions within neuron ensemble were significantly weakened (p < 0.01 in 24 h, p < 0.05 in 48 h), whereas no significant difference in 72 h (p > 0.05), which were consistent with the behavior results. These findings could lead to improved understanding of the mechanism of anesthesia inhibition on WM functions from the view of single neuron activity and neuron ensemble interactions. (C) 2014 Elsevier B.V. All rights reserved.