Trapping of DNA in nonuniform oscillating electric fields
Trapping of DNA in nonuniform oscillating electric fields
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DOI：
10.1016/s0006-3495(98)74027-5
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发表时间：
1998-02-01
影响因子：
3.4
通讯作者：
van den Engh, G
中科院分区：
文献类型：
--
作者：
Asbury, CL;van den Engh, G
DNA分子可以在水溶液中以类似于光学捕获的方式操纵。由于电偶极子的诱导，将DNA分子通过梯度力拉到高电场强度的区域。分子可以使用非常薄的黄金膜的条将局部捕获在振荡场中，以产生具有陡峭梯度的强电场。由于沿金膜条的边缘局限于与5 mu m相似的宽度，因此可以实现对捕获分子的空间控制。通过混合静态电场和振荡电场，可以将捕获的分子从一个边缘移到另一个边缘或沿边缘遵循精确的轨迹。该现象应在微型问题中有用，可用于操纵少量或单个DNA分子。
DNA molecules can be manipulated in aqueous solution in a manner analogous to optical trapping. Due to the induction of an electric dipole, DNA molecules are pulled by a gradient force to regions of high electric field strength. Molecules can be locally trapped in an oscillating field using strips of very thin gold film to generate strong electric fields with steep gradients. Spatial control over the trapped molecules is achieved because they are confined to a width of similar to 5 mu m along the edges of the gold-film strips. By mixing static and oscillating electric fields, trapped molecules can be moved from one edge to another or made to follow precise trajectories along the edges. This phenomenon should be useful in microdevices for manipulation of small quantities or single molecules of DNA.