Melatonin and light induce phase shifts of circadian activity rhythms in the C3H/HeN mouse
Melatonin and light induce phase shifts of circadian activity rhythms in the C3H/HeN mouse
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DOI：
10.1177/074873049601100204
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发表时间：
1996-06-01
影响因子：
3.5
通讯作者：
Dubocovich, ML
中科院分区：
文献类型：
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作者：
Benloucif, S;Dubocovich, ML
本研究考察了光脉冲和松果体激素褪黑激素对C3H/HEN小鼠昼夜活动节律的影响。小鼠被关在装有滚轮的笼子里，在持续的黑暗中。在用15分钟的光脉冲(300勒克斯)或给药(乙醇/生理盐水)或褪黑素(90微克，sc)治疗后，测量轮子运行活动的昼夜节律的相移。光处理导致昼夜活动节律的相变；具体地说，在主观早夜(昼夜时间[CT]12.5到CT18.5)期间延迟，在主观深夜(CT 0.5)提前。在不同的CT上单次注射褪黑素对自由奔跑的昼夜活动节律没有一致的影响。相反，在相同的时钟时间连续给药3天，当在CT10第一次给药时，昼夜活动节律的提前超过1h，当第一次给药在CT24和CT2之间时，引起昼夜活动节律的延迟长达1h。尽管需要多次褪黑素治疗才能诱导相移，但结果表明，控制C3H/HEN轮子活动节律的昼夜节律系统对光和褪黑素都有反应。
This study examines the effect of light pulses and administration of the pineal hormone melatonin on the circadian activity rhythm of C3H/HeN mice. Mice were housed in constant dark in cages equipped with running wheels. Phase shifts in the circadian rhythm of wheel-running activity were measured following treatment with a 15-min pulse of light (300 lux) or administration of vehicle (ethanol/saline) or melatonin (90 mu g, sc). Light treatment induced phase changes in circadian activity rhythms; specifically, delays during early subjective night (circadian time [CT] 12.5 to CT 18.5) and advances during late subjective night (CT 0.5). A single dose of melatonin administered at various CTs had no consistent effect on free-running circadian activity rhythms. By contrast, melatonin administration for 3 consecutive days at the same clock time induced advances in circadian activity rhythms by more than 1 h when the first dose was administered at CT 10 and induced delays in circadian activity rhythms by up to 1 h when the first dose was administered between CT 24 and CT 2. With the caveat that multi- ple melatonin treatments are required to induce phase shifts, the results suggest that the circadian timing system controlling the rhythm of wheel-running activity in the C3H/HeN mouse is responsive to both light and melatonin.