Lamprey buccal gland secretory protein-2 (BGSP-2) inhibits human T lymphocyte proliferation
Lamprey buccal gland secretory protein-2 (BGSP-2) inhibits human T lymphocyte proliferation
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DOI：
10.1093/czoolo/56.2.252
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发表时间：
2010-04
期刊：
影响因子：
2.2
通讯作者：
Jing;Sun;Shuiyan;Yu;Zhuang;Xue;Cenjie;Liu;Wu;Xin;Qingwei;Li
中科院分区：
文献类型：
--
作者：
Jing;Sun;Shuiyan;Yu;Zhuang;Xue;Cenjie;Liu;Wu;Xin;Qingwei;Li
七鳃鳗是无颌类的代表，无颌类是最古老的脊椎动物。寄生七鳃鳗从其颊腺分泌抗凝血剂，防止宿主鱼的血液凝固。从日本七鳃鳗（Lampetra japonica）颊腺cDNA文库中克隆到一个颊腺分泌蛋白2（BGSP-2）。克隆了BGSP-2全长基因，并获得了重组蛋白。通过检测BGSP-2对人T淋巴细胞的作用来研究其对淋巴细胞增殖的作用。我们发现七鳃鳗BGSP-2能够通过诱导G1/S期细胞阻滞而有效地阻断T细胞的体外增殖。在最低浓度为0.1μg/ml时，对植物血凝素（PHA）刺激的人T淋巴细胞增殖有抑制作用。我们的数据表明七鳃鳗BGSP-2能够在G1/S点阻断人T淋巴细胞的有丝分裂，并且对PHA活化的T淋巴细胞具有抗增殖作用的潜力（Current Zoology 56（2）：252-258，2010）。富含半胱氨酸的分泌蛋白（CRISP）超家族由大量具有不同进化起源和功能的单链分泌蛋白组成。CRISPs含有16个高度保守的半胱氨酸
Lamprey is a representative of the agnathans, the most ancient class of vertebrates. Parasitic lampreys secrete antico- agulant from their buccal glands and prevent blood coagulation of host fishes. We identified a buccal gland secretory protein-2 (BGSP-2) from a buccal gland cDNA library of Lampetra japonica. The full-length BGSP-2 gene was cloned and the recombi- nant BGSP-2 protein was generated. The role of BGSP-2 on lymphocyte proliferation was studied by examining its effects on human T lymphocytes. We found that lamprey BGSP-2 was able to effectively block the proliferation of T cells in vitro by induc- ing G1/S cell cycle arrest. Furthermore, it inhibited the proliferation of human T lymphocytes stimulated by phytohemagglutinin (PHA) at a minimum concentration of 0.1μg/ml. Our data suggest that lamprey BGSP-2 is able to block the mitosis of human T lymphocytes at the G1/S point, and has the potential of anti-proliferative effect on PHA-activated T lymphocytes (Current Zoology 56 (2): 252-258, 2010). The cysteine-rich secretory protein (CRISP) super- family comprises a large number of single-chain secre- tory proteins with different evolutionary origins and functions. CRISPs contain 16 highly conserved cysteine