Speed-mapping quantitative trait loci using microarrays
Speed-mapping quantitative trait loci using microarrays
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DOI：
10.1038/nmeth1084
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发表时间：
2007-10-01
期刊：
影响因子：
48
通讯作者：
Mackay, Trudy F. C.
中科院分区：
文献类型：
--
作者：
Lai, Chao-Qiang;Leips, Jeff;Mackay, Trudy F. C.
我们开发了一种快速、经济的方法，利用微阵列对混合DNA样本进行选择性基因分型，进行高分辨率的数量性状座位(QTL)定位。我们从两个近亲繁殖的黑腹果蝇品系中产生了21,207只F-2果蝇，这些品系的QTL已知会影响寿命，并将年轻果蝇和老年果蝇的DNA池杂交到芯片中。我们利用2326个单特征多态(SFP)基因频率的变化来定位先前识别的和影响寿命的额外QTL。
We developed a rapid, economical method for high-resolution quantitative trait locus ( QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F-2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays. We used changes of gene frequency of 2,326 single-feature polymorphisms ( SFPs) to map previously identified and additional QTLs affecting lifespan.