Identification and characterization of a novel spermatogenesis related gene LM23 in rat testis.
Identification and characterization of a novel spermatogenesis related gene LM23 in rat testis.
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DOI：
10.1016/j.bbrc.2007.03.024
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发表时间：
2007-05
影响因子：
3.1
通讯作者：
Mei-ling Liu;Kai-yan Pei-;Xin-quan Shi;De-yu Liu;M. Jia
中科院分区：
文献类型：
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作者：
Mei-ling Liu;Kai-yan Pei-;Xin-quan Shi;De-yu Liu;M. Jia
本研究旨在筛选与精子发生相关的新基因。利用差异显示PCR技术，从大鼠A型精原细胞、粗线期精母细胞和圆形精子细胞的总RNA中克隆到一个新的睾丸特异性基因LM 23。LM 23 cDNA全长1896 bp，开放阅读框为936 bp，编码312个氨基酸，与已知基因无明显同源性。LM 23的序列已提交至基因库，登录号为AF 492385。多组织北方杂交和RT-PCR分析表明，LM 23在睾丸中特异性表达，而在其它组织中未检测到表达。实时PCR分析表明，LM 23在精母细胞中的表达水平最高，而在精原细胞中的表达水平很低。原位杂交显示精母细胞胞质内呈强阳性信号，精子细胞和精原细胞内呈弱阳性信号。这些结果表明，LM 23具有睾丸特异性和阶段特异性表达特征，可能参与大鼠精子发生。
The aim of this study is to screen the novel gene related to the spermatogenesis. A novel rat testis-specific gene LM23 was identified and characterized by differential display PCR with total RNA from rat type A spermatogonia, pachytene spermatocytes, and round spermatids. LM23 cDNA consists of 1896 base pairs (bp) with a complete open reading frame of 936bp, and encodes a putative protein including 312 amino acids, which shares no significant homology with any known gene. The sequence of LM23 was submitted to GenBank and the Accession No. was AF492385. Multitissue Northern blot and RT-PCR analysis showed LM23 was specific expression in testis, while its expression was not detected in other tissues. Real-time PCR analysis showed that the expression level of LM23 was highest in spermatocytes and very low in spermatogonia. In situ hybridization revealed strong cytoplasmic positive signal in spermatocytes and weak signal in spermatids and spermatogonia. These results indicated LM23 possessed the testis-specific and stage-specific expression characteristics, and possibly involved in rat spermatogenesis.