Characterization of the nuclear import mechanisms of HSV-1 UL31
Characterization of the nuclear import mechanisms of HSV-1 UL31
复制标题
HSV-1 UL31 核输入机制的表征
DOI：
10.1515/hsz-2015-0299
复制
发表时间：
2016-06-01
影响因子：
3.7
通讯作者：
Li, Meili
中科院分区：
文献类型：
--
作者：
Cai, Mingsheng;Si, Jiang;Li, Meili
UL 31作为一种重要的蛋白质，在单纯疱疹病毒1型（HSV-1）的复制过程中发挥着多种作用。以往的研究表明，UL 31在化学固定细胞和活细胞中主要定位于细胞核，但其核转位的决定机制尚不清楚。在本研究中，利用活细胞荧光显微镜和免疫共沉淀分析，其特征在于，核输入的UL 31依赖于Ran-，importin α 1-和transportin-1-介导的途径。因此，这些结果将促进理解UL 31介导的HSV-1感染周期中的生物学功能。
As an important protein, UL31 has been demonstrated to play multiple roles in herpes simplex virus 1 (HSV-1) replication. Previous studies showed that UL31 predominantly locates in the nucleus in chemical fixed cells and live cells, however, the determining mechanisms for its nuclear translocation is not clear. In the present study, by utilizing live cells fluorescent microscopy and co-immunoprecipitation assays, the nuclear import of UL31 was characterized to be dependent on Ran-, importin alpha 1- and transportin-1-mediated pathway. Therefore, these results will promote the understanding of UL31-mediated biological functions in HSV-1 infection cycle.