Involvement of NO in fungal elicitor-induced activation of PAL and stimulation of taxol synthesis inTaxus chinensis suspension cells
Involvement of NO in fungal elicitor-induced activation of PAL and stimulation of taxol synthesis inTaxus chinensis suspension cells
复制标题
DOI：
10.1007/bf03184034
复制
发表时间：
2004-05
影响因子：
--
通讯作者：
Maojun Xu;Jufang Dong;Mu-yuan Zhu
中科院分区：
文献类型：
--
作者：
Maojun Xu;Jufang Dong;Mu-yuan Zhu
从黄霉青霉细胞壁制备的激发子诱导红豆杉细胞产生一氧化氮（NO），随后激活PAL并合成紫杉醇。一氧化氮清除剂cPITO和一氧化氮合酶（NOS）抑制剂PBITU阻止后两种反应，这两种反应都是在没有激发剂的情况下由一氧化氮供体硝普钠（SNP）触发的。PBITU对诱导剂诱导的红豆杉悬浮细胞NO释放有较强的抑制作用。上述结果表明，NO的产生与诱导子对红豆杉细胞后两种反应之间存在一定的因果关系，同时表明在真菌诱导子处理下，红豆杉细胞通过NOS产生的NO可能是触发PAL活化和紫杉醇合成的重要信号分子。
Elicitor prepared from the cell walls ofPenicillium citrinuminduces multiple responses ofTaxus chinensiscells, including nitric oxide (NO) generation, sequentially followed by the activation of PAL and synthesis of taxol. NO scavenger cPITO and nitric oxide synthase (NOS) inhibitor PBITU prevent the latter two reactions, all of which are triggered in the absence of elicitor by NO donor sodium nitroprusside (SNP). The elicitor-induced NO release ofTaxus chinensissuspension cells is strongly inhibited by PBITU. These results demonstrate a causal relationship between NO generation and the latter two reactions ofTaxus chinensiscells to the elicitor, and also indicate that NO, produced via NOS inTaxus chinensiscells treated with fungal elicitor, might act as an essential signaling molecule for triggering the activation of PAL and synthesis of taxol.