A novel approach based on low-field NMR for the detection of the pathological components of sputum in cystic fibrosis patients
A novel approach based on low-field NMR for the detection of the pathological components of sputum in cystic fibrosis patients
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DOI：
10.1002/mrm.26876
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发表时间：
2018-04-01
影响因子：
3.3
通讯作者：
Grassi, Mario
中科院分区：
文献类型：
--
作者：
Abrami, Michela;Ascenzioni, Fiorentina;Grassi, Mario
目的建立一种可靠、简便的监测囊性纤维化（CF）患者肺部状况的方法。CF患者的肺功能评估是相关的，因为他们中的大多数仍然死于呼吸衰竭。在CF患者的肺粘液（痰）中，诸如蛋白质、生物聚合物、DNA、细菌和粘蛋白的组分病理性地增加。由于肺功能与痰中的病理成分的量有关，它们的测定可以帮助临床医生监测肺部状况和规划therapy.MethodsLow-field NMR被用来评估CF痰中存在的水氢的弛豫时间（T-2 m）的变化与病理成分的量的关系。在人工样品（粘蛋白或藻酸盐）中测试低场NMR，然后在条件性痰液（来自健康志愿者的唾液，添加不同量的病理组分）中测试，最后在12名患者的唾液中测试，其中T-2 m与常用的肺监测测试（即，第1秒用力呼气量（FVC）。结果T（2 m）显著（P
PurposeDevelopment of a reliable, simple method to monitor lung condition in cystic fibrosis (CF) patients. Lung functionality assessment in CF patients is relevant, as most of them still die of respiratory failure. In lung mucus (sputum) of CF patients, components such as proteins, biopolymers, DNA, bacteria, and mucin are pathologically increased. As lung functionality is related to the amount of the pathological components in the sputum, their determination can help clinicians in monitoring lung condition and planning therapy.MethodsLow-field NMR was used to evaluate the variation of the relaxation time (T-2m) of the water hydrogens present in CF sputum in relation to the amounts of the pathological components. Low-field NMR was tested in artificial samples (mucin or alginates), then in conditional sputum (saliva from healthy volunteers, added by different amounts of the pathological components), and finally in 12 patients' sputums, in which T-2m was correlated to a commonly used lung monitoring test (i.e., forced expiratory volume in the first second).ResultsT(2m) significantly (P