Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats
Anti-apoptotic and neuroprotective effects of edaravone following transient focal ischemia in rats
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DOI：
10.1016/j.ejphar.2005.04.036
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发表时间：
2005-06-01
影响因子：
5
通讯作者：
Katayama, Y
中科院分区：
文献类型：
--
作者：
Amemiya, S;Kamiya, T;Katayama, Y
为了研究抗氧化剂依达拉奉对凋亡过程的影响，我们检测了Bax和Bcl-2的免疫组织化学表达和末端脱氧核苷酸转移酶介导的dUTP-生物素缺口末端标记（TUNEL）反应性。采用大鼠大脑中动脉阻断2 h的方法制备局灶性脑缺血模型。再灌注后静脉注射依达拉奉或生理盐水。再灌注24小时后，进行梗死体积、Bax和Bcl-2免疫组化和TUNEL染色以及神经功能评价。治疗组之间的皮质脑血流量无统计学差异。依达拉奉治疗的动物显示出显著改善的神经学结果。依达拉奉组的总梗死体积和皮质梗死体积显著减少。此外，依达拉奉治疗可显著减少梗死周围区域内TUNEL阳性凋亡细胞的数量，降低Bax免疫反应性，增加Bcl-2表达。依达拉奉通过Bax/Bcl-2依赖的抗凋亡机制对缺血/再灌注脑损伤具有良好的神经保护作用。(c)2005 Elsevier B.V保留所有权利。
To investigate the effect of an antioxidant edaravone on the apoptotic process, we examined Bax and Bcl-2 immunohistochemical expression and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) reactivity. Rat focal ischemia models were prepared by 2 h transient middle cerebral artery occlusion. Edaravone or physiological saline was intravenously administered after reperfusion. After 24 It of reperfusion, infarction volume assessments, Bax and Bcl-2 immunohistochemistry and TUNEL staining were performed as well as neurological evaluation. Cortical cerebral blood flow was not statistically different between the treatment-groups. Edaravone-treated animals showed significantly improved neurological outcome. Total and cortical infarct volumes in the edaravone group significantly decreased. In addition, edaravone-treatment provided a significant reduction in the number of TUNEL-positive apoptotic cells, a decrease in Bax immunoreactivity and an increase in Bcl-2 expression within the peri-infarct area. Edaravone shows an excellent neuroprotective effect against ischemia/reperfusion brain injury through a Bax/Bcl-2 dependent antiapoptotic mechanism. (c) 2005 Elsevier B.V All rights reserved.