Cytokines as mediators for or effectors against rotavirus disease in children
Cytokines as mediators for or effectors against rotavirus disease in children
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DOI：
10.1128/cdli.10.6.995-1001.2003
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发表时间：
2003-11-01
期刊：
影响因子：
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通讯作者：
Glass, RI
中科院分区：
文献类型：
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作者：
Jiang, B;Snipes-Magaldi, L;Glass, RI
轮状病毒是幼儿严重胃肠炎最常见的病因，但该病的发病机制和免疫机制尚未完全清楚。为了检测宿主对急性感染的反应，我们收集了30例轮状病毒腹泻患儿的配对血清标本，并使用基于微球的Luminex FlowerTM系统测量了9种细胞因子（白细胞介素-1 β [IL-1 β]、IL-2、IL-4、IL-6、IL-8、IL-10、IL-12、γ干扰素[IFN-γ]和肿瘤坏死因子α [TNF-α]）的水平。急性轮状病毒感染患者血清中7种细胞因子的中位数水平升高，其中3种（IL-6、IL-10和IFN-γ）的水平显著高于（P < 0.05）无腹泻的对照儿童的血清水平。发热组患儿血清IL-6水平显著高于对照组（P < 0.05），发热伴腹泻组患儿血清TNF-α水平显著高于无发热伴腹泻组（P < 0.05）。我们进一步证明了IL-2水平与第一次血样采集当天的粪便数量之间的负相关性（P < 0.05）。呕吐组IFN-γ水平显著低于无呕吐组（P < 0.05）。我们的初步研究提供的证据表明，在儿童轮状病毒感染的细胞因子反应的类型和幅度的影响或反映疾病的临床结果。这些研究结果表明，某些细胞因子可能在儿童轮状病毒病的发病机制和预防中发挥重要作用，因此，可能提供方向和见解，可能对预防或治疗这一重要疾病至关重要。
Rotavirus is the most common cause of severe gastroenteritis in young children, but the pathogenesis and immunity of this disease are not completely understood. To examine the host response to acute infection, we collected paired serum specimens from 30 children with rotavirus diarrhea and measured the levels of nine cytokines (interleukin-1beta [IL-1beta], IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, gamma interferon [IFN-gamma], and tumor necrosis factor alpha [TNF-alpha]) using a microsphere-based Luminex Flowmetrix system. Patients with acute rotavirus infection had elevated median levels of seven cytokines in serum, and of these, the levels of three (IL-6, IL-10, and IFN-gamma) were significantly (P < 0.05) higher than those in serum from control children without diarrhea. Patients with fever had significantly (P < 0.05) higher levels of IL-6 in serum than control children, and those with fever and more episodes of diarrhea had significantly (P < 0.05) higher levels of TNF-alpha than those without fever and with fewer episodes of diarrhea. We further demonstrated a negative association (P < 0.05) between the levels of IL-2 and the number of stools on the day on which the first blood sample was collected. Finally, patients with vomiting had significantly (P < 0.05) lower levels of IFN-gamma than those without vomiting. Our pilot study provides evidence that the types and magnitudes of cytokine responses to rotavirus infection in children influence or reflect the clinical outcome of disease. These findings suggest that certain cytokines may play an important role in the pathogenesis of and the protection against rotavirus disease in children and, consequently, may provide directions and insights that could prove critical to the prevention or treatment of this important disease.