MICROVASCULAR BASAL LAMINA ANTIGENS DISAPPEAR DURING CEREBRAL-ISCHEMIA AND REPERFUSION
MICROVASCULAR BASAL LAMINA ANTIGENS DISAPPEAR DURING CEREBRAL-ISCHEMIA AND REPERFUSION
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DOI：
10.1161/01.str.26.11.2120
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发表时间：
1995-11-01
期刊：
影响因子：
8.3
通讯作者：
DELZOPPO, GJ
中科院分区：
文献类型：
--
作者：
HAMANN, GF;OKADA, Y;DELZOPPO, GJ
背景与目的血管通透性的改变是脑缺血的重要后果。水肿和点状出血的发生与血管完整性的改变有关。一个主要的一部分，在微血管的完整性发挥了基底lamina.Methods的命运基底层的成分层粘连蛋白，纤维连接蛋白，和IV型胶原蛋白在大脑中动脉闭塞（2小时，n = 3）和闭塞（3小时）与再灌注（1小时，n = 3; 4小时，n = 3;和24小时，n = 4）在非人灵长类动物进行了评价。使用针对这些组分的特异性单克隆抗体。每个标本中的微血管的数量和大小分布通过视频成像显微镜测定，层粘连蛋白的相对荧光强度通过激光共聚焦显微镜进行半定量。基底层抗原介绍进行了比较双染色免疫荧光histochemistry.Results的微血管结构的数量定义的存在下，每个基底层抗原的再灌注24小时显着下降（P < .0001）。缺血区和非缺血区之间含层粘连蛋白血管的比率从对照组（0.98 ± 0.04）到缺血2小时（0.83 ± 0.09）和再灌注1小时（0.79 ± 0.08）、4小时（0.77 ± 0.06）和24小时（0.55 ± 0.07）显著降低。纤连蛋白（细胞）和含胶原（IV）血管的比率分别从0.98 +/- 0.04降至0.75 +/- 0.1和从1.02 +/- 0.03降至0.57 +/- 0.1。平均层粘连蛋白荧光强度从76.1 +/- 6.0 U（对照组）下降到52.0 +/- 14.6 U（再灌注24小时; P <0.001）。这些现象对于理解细胞外渗和急性中风的出血后果可能是重要的。
Background and Purpose Changes in vascular permeability are well-known and important consequences of cerebral ischemia mia. The development of edema and of petechial hemorrhage is connected to altered vascular integrity. A major part in microvascular integrity is played by the basal lamina.Methods The fates of the basal lamina components laminin, fibronectin, and type TV collagen during middle cerebral artery occlusion (2 hours, n = 3) and occlusion (3 hours) with reperfusion (1 hour, n = 3; 4 hours, n = 3; and 24 hours, n = 4) were evaluated in the nonhuman primate. Specific monoclonal antibodies against these components were used. The number and size distribution of the microvessels in each specimen were determined by video-imaging microscopy, and the relative fluorescence intensity of laminin was semiquantified by laser confocal microscopy. Basal lamina antigen presentations were compared by double-stain immunofluorescence histochemistry.Results The number of microvascular structures defined by the presence of each basal lamina antigen decreased significantly up to 24 hours of reperfusion (P < .0001). The ratio of laminin-containing vessels between the ischemic and nonischemic territories decreased significantly from control (0.98 +/- 0.04) to 2 hours of ischemia (0.83 +/- 0.09) and 1 hour (0.79 +/- 0.08), 4 hours (0.77 +/- 0.06), and 24 hours of reperfusion (0.55 +/- 0.07). The ratio of fibronectin (cellular) and of collagen (IV)-containing vessels decreased from 0.98 +/- 0.04 to 0.75 +/- 0.1 and from 1.02 +/- 0.03 to 0.57 +/- 0.1, respectively. Mean laminin fluorescence intensity decreased from 76.1 +/- 6.0 U (controls) to 52.0 +/- 14.6 U (24 hours of reperfusion; P < .001).Conclusions The significant parallel losses of three basal lamina components, both in number and intensity, contribute to loss of microvascular integrity. These phenomena may be important for understanding cell extravasation and the hemorrhagic consequences of acute stroke.