ELISA absorbance cut‐off method affects malaria sporozoite rate determination in wild Afrotropical Anopheles
ELISA absorbance cut‐off method affects malaria sporozoite rate determination in wild Afrotropical Anopheles
复制标题
ELISA吸光度截止法对野生非洲热带按蚊疟原虫子孢子率测定的影响
DOI：
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发表时间：
1988
影响因子：
1.9
通讯作者：
Joseph K. Koros
中科院分区：
文献类型：
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作者：
J. Beier;C. Asiago;Fred K. Onyango;Joseph K. Koros
抽象的。 244 只冈比亚按蚊和 115 只按蚊混合物种组中的疟疾子孢子感染率。使用三种方法对 funestus Giles 野生雌性蚊子进行比较，以确定恶性疟原虫 Welch 酶联免疫吸附测定 (ELISA) 阳性的截止吸光度值。阳性对照基于恶性疟原虫环子孢子蛋白。作为阴性对照，每个微量滴定板上包括四只野生雄性按蚊；每个板连续四天重复测试。
ABSTRACT. Malaria sporozoite infection rates in a mixed species group of 244 Anopheles gambiae Giles sensu lato and 115 An. funestus Giles wild female mosquitoes were compared using three methods to determine cutoff absorbance values for positivity of a Plasmodium falciparum Welch enzyme‐linked immunosorbent assay (ELISA). Positive controls were based on P. falciparum circumsporozoite protein. As negative controls, four wild male Anopheles were included on each microtitre plate; tests were repeated on four consecutive days for each plate.