Unusually Situated Binding Sites for Bacterial Transcription Factors Can Have Hidden Functionality.
Unusually Situated Binding Sites for Bacterial Transcription Factors Can Have Hidden Functionality.
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DOI：
10.1371/journal.pone.0157016
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Grainger DC
中科院分区：
文献类型：
--
作者：
Haycocks JR;Grainger DC
一个普遍接受的分子生物学范式是转录因子通过基因5‘端的结合位点来控制基因的表达。然而，越来越多的证据表明，转录因子靶标可以出现在基因内或聚合基因之间。在这项工作中，我们研究了肠毒素大肠埃希菌环状AMP受体蛋白(CRP)的一个这样的靶点。我们发现，CRP结合在两个趋同基因之间。当结合时，CRP调节一个小的开放阅读框架的转录，我们称之为AATS，嵌入到相邻的基因之一中。我们的工作表明，转录因子结合的非规范位点可以具有隐藏的功能。
A commonly accepted paradigm of molecular biology is that transcription factors control gene expression by binding sites at the 5' end of a gene. However, there is growing evidence that transcription factor targets can occur within genes or between convergent genes. In this work, we have investigated one such target for the cyclic AMP receptor protein (CRP) of enterotoxigenic Escherichia coli. We show that CRP binds between two convergent genes. When bound, CRP regulates transcription of a small open reading frame, which we term aatS, embedded within one of the adjacent genes. Our work demonstrates that non-canonical sites of transcription factor binding can have hidden functionality.