Glial cell-line derived neurotrophic factor-dependent fusimotor neuron survival during development.
Glial cell-line derived neurotrophic factor-dependent fusimotor neuron survival during development.
复制标题
神经胶质细胞系衍生的神经营养因子依赖性梭运动神经元在发育过程中的存活。
DOI：
10.1016/j.mod.2004.09.003
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Tourtellotte,WarrenG
中科院分区：
文献类型：
--
作者：
Whitehead,Jennifer;Keller-Peck,Cynthia;Kucera,Jan;Tourtellotte,WarrenG
胶质细胞源性神经营养因子(GDNF)是运动神经元的一种强有力的生存因子。以前的研究表明，一些运动神经元在发育过程中依赖于GDNF，但这种依赖GDNF的运动神经元亚群尚未被表征。我们研究了GDNF在肌肉中的表达模式，以及GDNF表达变化对运动神经元亚型发育的影响。在GDNF半合子小鼠中，对肌梭拉伸受体的运动神经元神经支配(融合运动神经元支配)减少，而在肌肉中过表达GDNF的转基因小鼠，对肌梭的融合运动神经支配增加。不含梭形运动神经元的面运动神经元，在发育过程中，当GDNF在面肌中过度表达时，其数量没有变化。综上所述，这些数据表明融合运动神经元在发育过程中依赖GDNF生存。由于GDNF缺陷小鼠出生时颈部和腰部运动神经元丢失的比例接近融合运动神经元池的大小，这些数据表明GDNF缺陷小鼠的运动神经元丢失可能主要源于融合运动神经元。
Glial cell-line derived neurotrophic factor (GDNF) is a potent survival factor for motor neurons. Previous studies have shown that some motor neurons depend upon GDNF during development but this GDNF-dependent motor neuron subpopulation has not been characterized. We examined GDNF expression patterns in muscle and the impact of altered GDNF expression on the development of subtypes of motor neurons. In GDNF hemizygous mice, motor neuron innervation to muscle spindle stretch receptors (fusimotor neuron innervation) was decreased, whereas in transgenic mice that overexpress GDNF in muscle, fusimotor innervation to muscle spindles was increased. Facial motor neurons, which do not contain fusimotor neurons, were not changed in number when GDNF was over expressed by facial muscles during their development. Taken together, these data indicate that fusimotor neurons depend upon GDNF for survival during development. Since the fraction of cervical and lumbar motor neurons lost in GDNF-deficient mice at birth closely approximates the size of the fusimotor neuron pool, these data suggest that motor neuron loss in GDNF-deficient mice may be primarily of fusimotor neuron origin.