Vertebrate lectins, Comparison of properties of beta-galactoside-binding lectins from tissues of calf and chicken.
Vertebrate lectins, Comparison of properties of beta-galactoside-binding lectins from tissues of calf and chicken.
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DOI：
10.1083/jcb.81.3.528
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发表时间：
1979-06
期刊：
影响因子：
--
通讯作者：
Kornfeld S
中科院分区：
文献类型：
--
作者：
Briles EB;Gregory W;Fletcher P;Kornfeld S
β-半乳糖苷结合凝集素从各种小牛组织和鸡心上的去唾液酸胎球蛋白-琼脂糖凝胶亲和层析分离，并进行了比较与生化特性，结合特性，抗原交叉反应性，和细胞定位。这些凝集素都是需要巯基的二价阳离子非依赖性二聚体，表观单体分子量为12，000（小牛凝集素）或13，000（鸡凝集素），酸性pI。小牛凝集素出现基本相同的十二烷基硫酸盐-聚丙烯酰胺凝胶电泳，等电聚焦，氨基酸组成，放射免疫分析，而鸡凝集素是明显不同的这些标准。然而，所有的凝集素竞争兔红细胞上相同的结合位点，并且可以被相同的糖半抗原（特别是乳糖和硫代二半乳糖苷）抑制。对几种培养细胞系的免疫荧光研究表明，牛和鸡凝集素主要存在于细胞内的细胞质中。脊椎动物的β-半乳糖苷结合凝集素似乎是种特异性的，而不是组织特异性的。
Beta-galactoside-binding lectins were isolated from various calf tissues and from chicken hearts by affinity chromatography on asialofetuin-Sepharose, and were compared with respect to biochemical characteristics, binding properties, antigenic cross-reactivity, and cellular localization. The lectins are all thiol group-requiring, divalent cation-independent dimers, of apparent monomer mol wt 12,000 (calf lectins) or 13,000 (chicken lectin), and acidic pI. The calf lectins appear essentially identical by dodecyl sulfate-polyacrylamide gel electrophoresis, isoelectric focusing, amino acid composition, and radioimmunoassay, while the chicken lectin is distinctly different by these criteria. However, all of the lectins competed for the same binding sites on rabbit erythrocytes, and could be inhibited by the same saccharide haptens (notably lactose and thiodigalactoside). Immuno- fluorescence studies on several cultured cell lines revealed that the bovine and chicken lectins had primarily an intracellular cytoplasmic localization. The beta-galactoside-binding lectins of vertebrates appear to be species-specific rather than tissue-specific.