Occurrence, molecular characterization and predominant genotypes of Enterocytozoon bieneusi in dairy cattle in Henan and Ningxia, China.
Occurrence, molecular characterization and predominant genotypes of Enterocytozoon bieneusi in dairy cattle in Henan and Ningxia, China.
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中国河南和宁夏奶牛比氏肠细胞虫的发生、分子特征及优势基因型
DOI：
10.1186/s13071-016-1425-5
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发表时间：
2016-03-11
影响因子：
3.2
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Li J;Luo N;Wang C;Qi M;Cao J;Cui Z;Huang J;Wang R;Zhang L
比氏肠细胞虫是人类和多种动物中最常见的微孢子虫。本研究旨在评估大肠埃希菌的患病率和分子特征。在中国中部的河南省和西北部的宁夏回族自治区的奶牛bieneusi。879份新鲜粪便标本中，24.3%（214/879）检出E.利用套式聚合酶链反应（PCR）技术，以内转录间隔区（ITS）基因为基础，对bieneusi的DNA序列进行了分析。在宁夏的一组腹泻奶牛中观察到最高感染率，为46.8%（51/109，P 0.0001）。感染率较高的年龄组为断奶前小牛（29.3%，127/434，P 0.0001）和断奶后小牛（23.9%，63/264，P = 0.006）。序列测定和系统发育分析表明，20株E. bieneusi ITS基因型（15个已知的和5个新的），包括组1和组2的成员。64.5%（138/214）的大肠杆菌中存在I和J基因型. bieneusi阳性标本。在本研究中，I型和J型是奶牛的优势基因型。人畜共患E.奶牛场的bieneusi表明牛可能作为人畜共患感染的储存宿主发挥重要作用。
Enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and a wide range of animals. This study was conducted to assess the prevalence and molecular characteristics of E. bieneusi in dairy cattle in Henan Province of central China and the Ningxia Hui Autonomous Region of northwest China. Of 879 fresh fecal specimens, 24.3 % (214/879) tested positive for E. bieneusi by nested polymerase chain reaction (PCR) based on the internal transcriber spacer (ITS) gene. The highest infection rate, 46.8 % (51/109, P < 0.0001), was observed in a group of dairy cattle with diarrhea, located in Ningxia. The age groups with higher infection rates were pre-weaned calves (29.3 %, 127/434, P < 0.0001) and post-weaned calves (23.9 %, 63/264, P = 0.006). Sequencing and phylogenetic analysis revealed 20 E. bieneusi ITS genotypes (15 known and five new), including members of Group 1 and Group 2. Genotypes I and J were detected in 64.5 % (138/214) of the E. bieneusi positive specimens. Genotypes I and J were the dominant genotypes in dairy cattle in the present study. The detection of zoonotic genotypes of E. bieneusi in dairy farms indicates that cattle may play an important role as a reservoir host for zoonotic infections.