Programmed cell death is affected in the novel mouse mutant Fused toes (Ft).
Programmed cell death is affected in the novel mouse mutant Fused toes (Ft).
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DOI：
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发表时间：
1994-09
期刊：
影响因子：
4.6
通讯作者：
F. Hoeven;T. Schimmang;A. Volkmann;M. Mattei;B. Kyewski;U. Rüther
中科院分区：
文献类型：
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作者：
F. Hoeven;T. Schimmang;A. Volkmann;M. Mattei;B. Kyewski;U. Rüther
我们已经确定了一种新的显性小鼠突变体，其特征是前肢脚趾融合和胸腺增生，在杂合动物中。突变的纯合性导致发育中的大脑畸形，失去左右不对称的遗传控制，并在发育的第10天左右死亡。体外对未成熟胸腺细胞肢体发育和诱导凋亡的分析表明，这种突变影响了细胞程序性死亡。由于突变是通过转基因插入引起的，我们能够将其定位到小鼠8号染色体的D区。到目前为止，没有任何影响程序性细胞死亡的突变被映射到这条染色体上。因此，这种突变将允许鉴定在哺乳动物发育过程中参与程序性细胞死亡的新基因。
We have identified a novel dominant mouse mutant that is characterised by fused toes on the fore limbs and a thymic hyperplasia, in heterozygous animals. Homozygosity of the mutation leads to malformation of the developing brain, lost of the genetic control of left-right asymmetry and to death around day 10 of development. Analysis of both limb development and induction of apoptosis in immature thymocytes in vitro suggest that programmed cell death is affected by the mutation. Since the mutation was caused via a transgene insertion we were able to map it to the D region on mouse chromosome 8. So far, no mutation that affects programmed cell death has been mapped to this chromosome. Thus, this mutation will allow the identification of a novel gene involved in programmed cell death during mammalian development.