INDUCED BIOCHEMICAL MUTATIONS IN BACILLUS-SUBTILIS
INDUCED BIOCHEMICAL MUTATIONS IN BACILLUS-SUBTILIS
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DOI：
10.2307/2437147
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发表时间：
1947-01-01
影响因子：
3
通讯作者：
GILES, NH
中科院分区：
文献类型：
--
作者：
BURKHOLDER, PR;GILES, NH
用U-V辐照枯草杆菌孢子或营养细胞。或者X射线导致了许多生化突变菌株的产生，这些突变菌株与亲本菌株不同，因为它们无法合成特定的生长因子。在4260个菌株中，已鉴定出143个单缺陷突变株。在3个一般分类类别中的每一个类别中出现的突变数量如下：33个菌株需要特定的B-维生素；4个菌株需要核酸成分；10个(5个)需要氨基酸。被测试的单缺陷突变体的生长与AMT成正比。所提供的生长物质。获得了几株具有多重要求的菌株。9个需要30摄氏度核黄素的独立突变菌株被发现是温度不敏感的类型，因为这些菌株也需要较低温度的维生素。突变株的产量相对较高，这一结果支持枯草杆菌孢子是单核单倍体的观点。
Irradiation of spores or vegetative cells of B. subtilis with either u.-v. or x-ray has resulted in the production of numerous biochemical mutant strains differing from the parent strain in their inability to synthesize specific growth factors. From 4260 isolates, 143 singly deficient mutants have been identified. The number of mutants occurring in each of the 3 general classification categories is as follows: 33 strains requiring specific B-vitamins; 4 requiring nucleic acid components; and 10(5 requiring amino acids. The growth of the singly deficient mutants which have been tested is proportional to the amt. of the growth substance supplied. A few isolates having multiple requirements have been obtained. 9 independent mutant strains requiring riboflavin at 30[degree]C were found to be temp.-insensitive types, since these strains also require the vitamin at lower temps. The relatively high yield of mutants which was obtained with irradiation and immediate plating of spores supports the view that the spores of B. subtilis are uninucleate and haploid.