Physiological Effects of Gibberellic Acid. VII. Electron Microscopy of Barley Aleurone Cells.
Physiological Effects of Gibberellic Acid. VII. Electron Microscopy of Barley Aleurone Cells.
复制标题
赤霉酸的生理作用。
DOI：
10.1104/pp.39.4.673
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发表时间：
1964
期刊：
影响因子：
7.4
通讯作者：
B. Hyde
中科院分区：
文献类型：
--
作者：
L. Paleg;B. Hyde
早期的工作表明，大麦胚乳，从胚中释放出来，响应赤霉酸（GA 3）处理，伴随着还原糖和蛋白质氮的释放（19，20，21）的干重损失。这些结果导致的结论是，胚和GA 3对胚乳的影响是相同的，并建议内源赤霉素可能是在胚中产生的激素，启动胚乳储备的崩溃。在试图追踪GA 3在大麦胚乳中的作用的过程中，发现糊粉层是直接受影响的组织（23）。从含淀粉胚乳上剥离的糊粉层组织的分离片段已经证明了作为GA 3处理的结果产生和释放α-淀粉酶的能力。结果提示，正是这种淀粉酶，因为它从完整发芽种子中的产生部位扩散，通过作用于胚乳内部的淀粉而引起还原糖的释放。糊粉层作为GA 3作用的细胞位点的鉴定，以及其分离技术的发展使得在视觉上跟踪GA 3诱导的该组织内的变化成为可能。本报告涉及GA 3处理的超微结构表现，用电子显微镜观察。初步报告已在其他地方提出（12）。
Earlier work indicated that barley endosperm, freed from the embryo, responds to gibberellic acid (GA3) treatment with a loss in dry weight accompanied by release of reducing sugars and protein nitrogen ( 19, 20, 21). These results lead to the conclusion that the effects of the embryo and GA3 on the endosperm are identical, and suggest that endogenous gibberellin may be the hormone produced in the embryo which initiates the breakdown of endospermal reserves. In an attempt to trace the action of GA3 to a locus within the barley endosperm it was shown that the aleurone layer was the tissue directly affected (23). Isolated segments of aleurone tissue, peeled away from the starchy endosperm, have demonstrated the ability to produce and release a-amylase as a result of GA3 treatment. The results prompted the conclusion that it is this amylase, as it diffuses from its site of production in the intact germinating seed which causes the release of reducing sugar by acting on starch in the inner portion of the endosperm. Identification of the aleurone layer as the cellular locus of GA3 action, and the development of techniques for its isolation have made it possible to follow, visually, the GA3-induced changes within this tissue. This report deals with ultrastructural manifestations of GA3 treatment, as observed with the electron microscope. A preliminary report has been presented elsewhere (12).