The prey’s scent – Volatile organic compound mediated interactions between soil bacteria and their protist predators
The prey’s scent – Volatile organic compound mediated interactions between soil bacteria and their protist predators
复制标题
DOI：
10.1038/ismej.2016.144
复制
发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Kristin Schulz-Bohm;S. Geisen;ER Jasper Wubs;Chunxu Song;W. D. Boer;P. Garbeva
中科院分区：
文献类型：
--
作者：
Kristin Schulz-Bohm;S. Geisen;ER Jasper Wubs;Chunxu Song;W. D. Boer;P. Garbeva
原生生物是土壤中细菌的主要捕食者。然而，目前尚不清楚原生生物如何在这个高度复杂的环境中感知猎物。在这里，我们研究了六种系统发育不同的土壤细菌的挥发性有机化合物（VOC）是否影响三种不同土壤原生生物的性能，以及它与直接摄食相互作用的关系。我们观察到大多数细菌受挥发性有机化合物影响原生生物活性。然而，原生生物对挥发性有机化合物的反应强烈依赖于细菌和原生生物的相互作用。挥发物对原生生物活性的刺激和直接营养相互作用测定通常是一致的，这表明挥发性有机化合物可以作为原生生物感知合适猎物的信号。此外，细菌萜烯合酶突变体失去了影响原生生物的能力，表明萜烯代表了 VOC 介导的通讯的关键组成部分。总的来说，我们证明挥发物直接参与原生生物-细菌捕食者-猎物的相互作用。
Protists are major predators of bacteria in soils. However, it remains unknown how protists sense their prey in this highly complex environment. Here, we investigated whether volatile organic compounds (VOCs) of six phylogenetic distinct soil bacteria affect the performance of three different soil protists and how that relates to direct feeding interactions. We observed that most bacteria affected protist activity by VOCs. However, the response of protists to the VOCs was strongly dependent on both the bacterial and protist interacting partner. Stimulation of protist activity by volatiles and in direct trophic interaction assays often coincided, suggesting that VOCs serve as signals for protists to sense suitable prey. Furthermore, bacterial terpene synthase mutants lost the ability to affect protists, indicating that terpenes represent key components of VOC-mediated communication. Overall, we demonstrate that volatiles are directly involved in protist−bacterial predator−prey interactions.