Analysis of genetic diversity in Arrhenatherum elatius Germplasm using inter-simple sequence repeat (ISSR) markers
Analysis of genetic diversity in Arrhenatherum elatius Germplasm using inter-simple sequence repeat (ISSR) markers
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DOI：
10.5897/ajb11.830
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发表时间：
2011-07
影响因子：
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通讯作者：
L. Meng;Hong Yang;P. Mao;H. Gao;F. Sun
中科院分区：
文献类型：
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作者：
L. Meng;Hong Yang;P. Mao;H. Gao;F. Sun
利用100个简单重复序列（inter-simple sequence repeat，ISSR）引物对19份高杆Arrhytherum elatius种质资源进行了遗传多样性分析，其中11个引物扩增产物差异显著。共检测到152条谱带，其中多态性谱带107条。多态性条带百分率（PPB）为68.9%，平均每个引物有9.73条多态性条带。19份材料间的遗传相似系数（GS）在0.4821 ~ 0.7411之间，显示出较高的遗传多样性。通过UPGMA聚类分析和主成分分析，对19个A. elatius种质分为三组，情况相似。发现19个A. elatius加入研究。这些结果证实了遗传多样性保护对未来育种计划的潜在价值。
The genetic diversity of 19 Arrhenatherum elatius accessions was analyzed using 100 inter-simple sequence repeat (ISSR) primers, out of which 11 generated distinct amplification products. Out of the 152 total bands detected, 107 were polymorphic. The percentage of polymorphic bands (PPB) was 68.9% with an average of 9.73 polymorphic bands per primer. The ISSR-based genetic similarity (GS) coefficients among the 19 accessions ranged from 0.4821 to 0.7411, revealing high genetic diversity. Based on the UPGMA cluster analysis and the principal components analysis (PCA), the 19 A. elatius accessions were divided into three groups with similar situations. We found that the genetic distance was related to the geographical distance among the 19 A. elatius accessions studied. These results confirm the potential value of genetic diversity preservation for future breeding programs.