Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
Expression and strain variation of the novel "small open reading frame" (smorf) multigene family in Babesia bovis
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DOI：
10.1016/j.ijpara.2011.10.004
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
McElwain, Terry F.
中科院分区：
文献类型：
--
作者：
Ferreri, Lucas M.;Brayton, Kelly A.;McElwain, Terry F.
小开放阅读框（smorf）基因是第二大的牛巴氏杆菌多基因家族。所有已知的44种变异smorf基因都位于染色体上与vesl基因接近的位置，vesl基因编码介导细胞粘附并有助于免疫逃避的蛋白质。在这项研究中，我们的特点是smorf基因的一般拓扑结构，并研究了基因库，转录谱和SMORF表达在两个不同的菌株，T2Bo和Mo7。使用简并引物的序列分析确定了额外的smorf基因在每个菌株中，并证明了smorf基因库不同菌株之间，在这两个保守和独特的基因。Smorf基因具有多个半保守和可变区，并且在44个基因中的20个中的大的高变插入定义了该家族的两个主要分支，称为smorf A和smorf B。在T2 Bo菌株B中，总共有32个smorf基因同时转录。从急性感染动物的脑深部组织中获得的牛裂殖子。在免疫印迹中，SMORF肽特异性抗血清与多种蛋白质结合，其大小范围由smorf基因预测，证实了来自这些转录物的smorf基因产物的翻译。这些结果表明，smorf多基因家族比以前描述的更大，并表明smorf基因的表达和正在经历的变化，无论是在菌株内，并在一个独立的菌株特异性的谱系特异性模式。这些新蛋白的功能尚不清楚。(C)2011年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Small open reading frame (smorf) genes comprise the second largest Babesia bovis multigene family. All known 44 variant smorf genes are located in close chromosomal proximity to vesl genes, which encode proteins that mediate cytoadhesion and contribute to immune evasion. In this study, we characterised the general topology of smorf genes and investigated the gene repertoire, transcriptional profile and SMORF expression in two distinct strains, T2Bo and Mo7. Sequence analysis using degenerate primers identified additional smorf genes in each strain and demonstrated that the smorf gene repertoire varies between strains, with conserved and unique genes in both. Smorf genes have multiple semi-conserved and variable blocks, and a large hypervariable insertion in 20 of the 44 genes defines two major branches of the family, termed smorf A and smorf B. A total of 32 smorf genes are simultaneously transcribed in T2Bo strain B. bovis merozoites obtained from deep brain tissue of an acutely infected animal. SMORF peptide-specific antiserum bound in immunoblots to multiple proteins with a range of sizes predicted by smorf genes, confirming translation of smorf gene products from these transcripts. These results indicate that the smorf multigene family is larger than previously described and demonstrate that smorf genes are expressed and are undergoing variation, both within strains and in a lineage-specific pattern independent of strain specificity. The function of these novel proteins is unknown. (C) 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.